Isolation of polymorphic microsatellite markers for the demersal fish Helicolenus dactylopterus (Dela Roche 1809)
Isolation of polymorphic microsatellite markers for the demersal fish Helicolenus dactylopterus (Dela Roche 1809)
Six microsatellite loci were identified for the demersal deep-sea fish Helicolenus dactylopterus. All loci were highly polymorphic (5–21 alleles per locus). Observed heterozygosities were from 0.378 to 0.868, while the expected heterozygosities ranged from 0.529 to 0.925. Multiplex PCR reactions were optimized. Microsatellite markers were developed for analysis of genetic structure including identification of stocks and migration patterns. The resulting data will be used to help in the establishment of scientifically based fisheries management for this species. Departures from the expected Hardy–Weinberg equilibrium were observed in three loci, and are likely to be a consequence of population structuring across the Azorean islands.
18-20
Aboim, M.A.
20fce01d-b1a7-4cdd-b613-fa155492e49d
Rogers, A.D.
906fd860-72c9-4e72-ba43-36e78a1f4037
Menezes, G.M.
20e751a6-c608-4c44-a8d8-35e28c369fb7
Maggioni, R.
e0a2e1f9-8531-4cc1-a544-11eb4a643b10
Pearson, C.V.M.
01cdc5f0-5ac7-462a-8fd8-3eb95ebc57de
14 December 2002
Aboim, M.A.
20fce01d-b1a7-4cdd-b613-fa155492e49d
Rogers, A.D.
906fd860-72c9-4e72-ba43-36e78a1f4037
Menezes, G.M.
20e751a6-c608-4c44-a8d8-35e28c369fb7
Maggioni, R.
e0a2e1f9-8531-4cc1-a544-11eb4a643b10
Pearson, C.V.M.
01cdc5f0-5ac7-462a-8fd8-3eb95ebc57de
Aboim, M.A., Rogers, A.D., Menezes, G.M., Maggioni, R. and Pearson, C.V.M.
(2002)
Isolation of polymorphic microsatellite markers for the demersal fish Helicolenus dactylopterus (Dela Roche 1809).
Molecular Ecology Notes, 3 (1), .
(doi:10.1046/j.1471-8286.2003.00337.x).
Abstract
Six microsatellite loci were identified for the demersal deep-sea fish Helicolenus dactylopterus. All loci were highly polymorphic (5–21 alleles per locus). Observed heterozygosities were from 0.378 to 0.868, while the expected heterozygosities ranged from 0.529 to 0.925. Multiplex PCR reactions were optimized. Microsatellite markers were developed for analysis of genetic structure including identification of stocks and migration patterns. The resulting data will be used to help in the establishment of scientifically based fisheries management for this species. Departures from the expected Hardy–Weinberg equilibrium were observed in three loci, and are likely to be a consequence of population structuring across the Azorean islands.
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Published date: 14 December 2002
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Local EPrints ID: 52513
URI: http://eprints.soton.ac.uk/id/eprint/52513
ISSN: 1471-8278
PURE UUID: 2d63901d-3ff0-445b-a85b-771bc0f2eb1b
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Date deposited: 03 Jul 2008
Last modified: 15 Mar 2024 10:37
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Author:
M.A. Aboim
Author:
A.D. Rogers
Author:
G.M. Menezes
Author:
R. Maggioni
Author:
C.V.M. Pearson
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