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Antimicrobial-resistance of Streptococcus pneumoniae isolated from the Lebanese environment

Record type: Article

The aim of this study was to isolate and molecularly characterize Streptococcus pneumoniae in samples collected from the aquatic environment in Lebanon. For this purpose, 57 samples of ground waters, sea water, sediments and crabs were collected. Biochemical tests as well as PCR were used for further identification and molecular characterization of 46 suspected isolates using specific primers for the lytA-gene and protein 2B-gene. The confirmed isolates were evaluated for their antimicrobial resistance, using the minimum inhibitory concentration method. Crab samples exhibited a high contamination level of an average count of 8.2 × 104 CFU/g. In water samples, the highest contamination was recorded near a coastal landfill with a bacterial count of 1.8 × 103 CFU/ml whereas the lowest bacterial counts (non detectable) were observed in ground water samples. Twenty out of the 46 suspected isolates came from sea water, and 17 out of those were characterized by PCR as S. pneumoniae. Testing the antimicrobial resistance of the 20 isolates to various antimicrobials showed that all sea water isolates were resistant to vancomycin, gentamicin, and oxacillin (100%), whereas 62.5% were resistant to clindamycin, and only 25% were erythromycin resistant. Resistance rates were higher in the case of fresh water samples, with 57% resistance to erythromycin, and 100% resistance to vancomycin, gentamicin, clindamycin and oxacillin. Alternatively, all tested crab isolates were susceptible to erythromycin. However, they showed a high resistance of 80% against vancomycin, gentamicin, clindamycin and 100% against oxacillin

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Citation

Harakeh, Steve, Yassine, Hadi and El-Fadel, Mutasem (2006) Antimicrobial-resistance of Streptococcus pneumoniae isolated from the Lebanese environment Marine Environmental Research, 62, (3), pp. 181-193. (doi:10.1016/j.marenvres.2006.03.010).

More information

Published date: September 2006
Keywords: streptococcus pneumoniae, antimicrobial resistance, marine environment, fresh water environment, molecular characterization

Identifiers

Local EPrints ID: 52903
URI: http://eprints.soton.ac.uk/id/eprint/52903
ISSN: 0141-1136
PURE UUID: 5b5df970-5006-43a8-83c9-a3bc3159b9df

Catalogue record

Date deposited: 15 Jul 2008
Last modified: 17 Jul 2017 14:39

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Contributors

Author: Steve Harakeh
Author: Hadi Yassine
Author: Mutasem El-Fadel

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