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An evaluation of mixed species in-situ and ex-situ feeding assays: The altered response of Asellus aquaticus and Gammarus pulex

An evaluation of mixed species in-situ and ex-situ feeding assays: The altered response of Asellus aquaticus and Gammarus pulex
An evaluation of mixed species in-situ and ex-situ feeding assays: The altered response of Asellus aquaticus and Gammarus pulex
Mixed species feeding assays were undertaken with pollution sensitive (Gammarus pulex) and tolerant (Asellus aquaticus) macro-invertebrates during August 2003 and April 2004. The purpose of this study was to establish if a test animals' response is comparable during in-situ and ex-situ toxicity tests. Seven test sites were established along an undisclosed stream, which received leachate discharge from an unlined, disused UK landfill site. Sampling points A–B were upstream of the contamination, C was adjacent to the influx and D–G were downstream of the leachate discharge (at 100 m intervals). During the in-situ and ex-situ tests, 2-week-old male laboratory bred A. aquaticus and G. pulex were used as test animals. The animals were transplanted to the seven sampling points for the duration of the in-situ tests, whilst water samples from each site were returned to the laboratory for ex-situ testing. The results show that the animals' mortality and feeding rates followed similar trends during the in-situ and ex-situ tests, however, the animals' response was amplified during the in-situ tests. It was also observed that the effects were greater in April, compared to August that may be attributed to a higher frequency of rainfall during spring, which could have flushed a greater proportion of the contaminant load from the waste mass and as a consequence, higher levels of pollution may have leached into the stream from the landfill site. The study, therefore, concludes that in-situ toxicity tests are a more precise monitoring technique, in comparison to ex-situ assays.
Asellus aquaticus, ex-situ and in-situ feeding assays, Gammarus pulex, landfill leachate, receiving waters, toxicity tests
0160-4120
22-27
Bloor, M.C.
97ed259c-17a3-40b5-95c2-f3f4957c36d5
Banks, C.J.
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f
Bloor, M.C.
97ed259c-17a3-40b5-95c2-f3f4957c36d5
Banks, C.J.
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f

Bloor, M.C. and Banks, C.J. (2006) An evaluation of mixed species in-situ and ex-situ feeding assays: The altered response of Asellus aquaticus and Gammarus pulex. Environment International, 32 (1), 22-27. (doi:10.1016/j.envint.2005.04.004).

Record type: Article

Abstract

Mixed species feeding assays were undertaken with pollution sensitive (Gammarus pulex) and tolerant (Asellus aquaticus) macro-invertebrates during August 2003 and April 2004. The purpose of this study was to establish if a test animals' response is comparable during in-situ and ex-situ toxicity tests. Seven test sites were established along an undisclosed stream, which received leachate discharge from an unlined, disused UK landfill site. Sampling points A–B were upstream of the contamination, C was adjacent to the influx and D–G were downstream of the leachate discharge (at 100 m intervals). During the in-situ and ex-situ tests, 2-week-old male laboratory bred A. aquaticus and G. pulex were used as test animals. The animals were transplanted to the seven sampling points for the duration of the in-situ tests, whilst water samples from each site were returned to the laboratory for ex-situ testing. The results show that the animals' mortality and feeding rates followed similar trends during the in-situ and ex-situ tests, however, the animals' response was amplified during the in-situ tests. It was also observed that the effects were greater in April, compared to August that may be attributed to a higher frequency of rainfall during spring, which could have flushed a greater proportion of the contaminant load from the waste mass and as a consequence, higher levels of pollution may have leached into the stream from the landfill site. The study, therefore, concludes that in-situ toxicity tests are a more precise monitoring technique, in comparison to ex-situ assays.

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More information

Submitted date: 14 January 2005
Published date: January 2006
Additional Information: Bloor M. C., Banks C. J., (2006). "An evaluation of mixed species in-situ and ex-situ acute toxicity tests: the altered response of Asellus aquaticus and Gammarus pulex." Environment International, 32, 22-27
Keywords: Asellus aquaticus, ex-situ and in-situ feeding assays, Gammarus pulex, landfill leachate, receiving waters, toxicity tests

Identifiers

Local EPrints ID: 53698
URI: https://eprints.soton.ac.uk/id/eprint/53698
ISSN: 0160-4120
PURE UUID: 71166c8e-877c-4ba9-888d-c3cfce9a3be8

Catalogue record

Date deposited: 21 Jul 2008
Last modified: 13 Mar 2019 20:40

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