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Synthesis and properties of oligonucleotides containing a cholesterol thymidine monomer

Synthesis and properties of oligonucleotides containing a cholesterol thymidine monomer
Synthesis and properties of oligonucleotides containing a cholesterol thymidine monomer
Highly selective base-pair recognition makes DNA a suitable building block for orderly self-assembled structures. For some applications in nanotechnology DNA complexes need to be fixed onto surfaces. To fulfil this requirement on lipid membranes we have synthesised a thymidine monomer modified with a cholesterol moiety. Solution studies show that the melting temperature (Tm) of the duplex, with adjacent cholesterols on each strand, is much higher than that of the unmodified duplex.
cholesterol, nonotechnology, dna, fluorescence melting, self-assembly
1525-7770
785-794
Durand, Adeline
9eef6a77-9477-405d-a8e2-e0237b52c412
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Durand, Adeline
9eef6a77-9477-405d-a8e2-e0237b52c412
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Durand, Adeline and Brown, Tom (2007) Synthesis and properties of oligonucleotides containing a cholesterol thymidine monomer. Nucleosides, Nucleotides & Nucleic Acids, 26 (6 & 7), 785-794. (doi:10.1080/15257770701501534).

Record type: Article

Abstract

Highly selective base-pair recognition makes DNA a suitable building block for orderly self-assembled structures. For some applications in nanotechnology DNA complexes need to be fixed onto surfaces. To fulfil this requirement on lipid membranes we have synthesised a thymidine monomer modified with a cholesterol moiety. Solution studies show that the melting temperature (Tm) of the duplex, with adjacent cholesterols on each strand, is much higher than that of the unmodified duplex.

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More information

Published date: June 2007
Keywords: cholesterol, nonotechnology, dna, fluorescence melting, self-assembly

Identifiers

Local EPrints ID: 54287
URI: http://eprints.soton.ac.uk/id/eprint/54287
ISSN: 1525-7770
PURE UUID: 9f2f6ab6-ae64-4aa7-972e-acdaa93413ce

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Date deposited: 30 Jul 2008
Last modified: 15 Mar 2024 10:46

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Contributors

Author: Adeline Durand
Author: Tom Brown

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