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Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions

Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions
Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions
Stable triplexes have been generated under near-physiological conditions by the introduction of the C and T base analogues 3-methyl-2-aminopyridine-2 '-deoxyriboside and 5-(3-aminoprop-2-ynyl)-'-deoxyuridine into psoralen-conjugated triplex-forming oligonucleotides. After irradiation with UV light at 365 nm, photo-induced cross-linking of the TFO to double-helical DNA was observed by UV-melting analysis and fluorescence measurements.
psoralen, triplex, nucleosides, 2-aminopyridine, crosslinking
1525-7770
1005-1009
Li, Hong
44d51d43-1fe5-4d15-820d-22d302b9e1a7
Broughton-Head, Victoria J.
d094aa77-c889-4747-84d2-55542805c67c
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Li, Hong
44d51d43-1fe5-4d15-820d-22d302b9e1a7
Broughton-Head, Victoria J.
d094aa77-c889-4747-84d2-55542805c67c
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Li, Hong, Broughton-Head, Victoria J., Fox, Keith R. and Brown, Tom (2007) Photoinduced crosslinking of double-helical DNA by psoralen covalently linked to a triple helix-forming oligonucleotide under near-physiological conditions. Nucleosides, Nucleotides & Nucleic Acids, 26 (8 & 9), 1005-1009. (doi:10.1080/15257770701508554).

Record type: Article

Abstract

Stable triplexes have been generated under near-physiological conditions by the introduction of the C and T base analogues 3-methyl-2-aminopyridine-2 '-deoxyriboside and 5-(3-aminoprop-2-ynyl)-'-deoxyuridine into psoralen-conjugated triplex-forming oligonucleotides. After irradiation with UV light at 365 nm, photo-induced cross-linking of the TFO to double-helical DNA was observed by UV-melting analysis and fluorescence measurements.

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More information

Published date: August 2007
Keywords: psoralen, triplex, nucleosides, 2-aminopyridine, crosslinking
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Identifiers

Local EPrints ID: 54336
URI: http://eprints.soton.ac.uk/id/eprint/54336
DOI: doi:10.1080/15257770701508554
ISSN: 1525-7770
PURE UUID: cbe4628e-98cd-4fec-ad19-26606465e071
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 30 Jul 2008
Last modified: 16 Mar 2024 02:36

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Contributors

Author: Hong Li
Author: Victoria J. Broughton-Head
Author: Keith R. Fox ORCID iD
Author: Tom Brown

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