Functional properties of type I and type II cytochromes c(3) from Desulfovibrio africanus
Functional properties of type I and type II cytochromes c(3) from Desulfovibrio africanus
Type I cytochrome c3 is a key protein in the bioenergetic metabolism of Desulfovibrio spp., mediating electron transfer between periplasmic hydrogenase and multihaem cytochromes associated with membrane bound complexes, such as type II cytochrome c3. This work presents the NMR assignment of the haem substituents in type I cytochrome c3 isolated from Desulfovibrio africanus and the thermodynamic and kinetic characterisation of type I and type II cytochromes c3 belonging to the same organism. It is shown that the redox properties of the two proteins allow electrons to be transferred between them in the physiologically relevant direction with the release of energised protons close to the membrane where they can be used by the ATP synthase.
sulfate-reducing bacteria, electron transfer rates, axial ligand orientation, nife, hydrogenase, thermodynamic characterisation, electron-transfer proteins, hildenborough, energy transduction, desulfovibrio africanus, multicentre protein, cytochrome c3, tetraheme cytochromes, hmc operon, proton thruster, desulfuricans atcc 27774, subsp vulgaris
178-188
Paquete, C.M.
33d20dad-2d71-4a8d-bc17-7e351e4d080d
Pereira, P.M.
456a96f5-10bd-4448-b25f-b78cfde3265a
Catarino, T.
02e16379-0fda-4ecd-828c-e10bb0e36502
Turner, D.L.
bc0db1b4-5aa2-4b89-833e-8f0d31499159
Louro, R.O.
5473a453-e54d-4559-968b-abfdc1f36d67
Xavier, A.V.
bc590fd4-ed8f-4ad4-a189-b0fc6b63ff2b
2007
Paquete, C.M.
33d20dad-2d71-4a8d-bc17-7e351e4d080d
Pereira, P.M.
456a96f5-10bd-4448-b25f-b78cfde3265a
Catarino, T.
02e16379-0fda-4ecd-828c-e10bb0e36502
Turner, D.L.
bc0db1b4-5aa2-4b89-833e-8f0d31499159
Louro, R.O.
5473a453-e54d-4559-968b-abfdc1f36d67
Xavier, A.V.
bc590fd4-ed8f-4ad4-a189-b0fc6b63ff2b
Paquete, C.M., Pereira, P.M., Catarino, T., Turner, D.L., Louro, R.O. and Xavier, A.V.
(2007)
Functional properties of type I and type II cytochromes c(3) from Desulfovibrio africanus.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1767 (2), .
(doi:10.1016/j.bbabio.2007.01.012).
Abstract
Type I cytochrome c3 is a key protein in the bioenergetic metabolism of Desulfovibrio spp., mediating electron transfer between periplasmic hydrogenase and multihaem cytochromes associated with membrane bound complexes, such as type II cytochrome c3. This work presents the NMR assignment of the haem substituents in type I cytochrome c3 isolated from Desulfovibrio africanus and the thermodynamic and kinetic characterisation of type I and type II cytochromes c3 belonging to the same organism. It is shown that the redox properties of the two proteins allow electrons to be transferred between them in the physiologically relevant direction with the release of energised protons close to the membrane where they can be used by the ATP synthase.
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Published date: 2007
Keywords:
sulfate-reducing bacteria, electron transfer rates, axial ligand orientation, nife, hydrogenase, thermodynamic characterisation, electron-transfer proteins, hildenborough, energy transduction, desulfovibrio africanus, multicentre protein, cytochrome c3, tetraheme cytochromes, hmc operon, proton thruster, desulfuricans atcc 27774, subsp vulgaris
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Local EPrints ID: 54355
URI: http://eprints.soton.ac.uk/id/eprint/54355
ISSN: 0005-2728
PURE UUID: 85b5457b-b0f2-4eee-bb8b-4a1aed14d4f4
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Date deposited: 31 Jul 2008
Last modified: 15 Mar 2024 10:47
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Contributors
Author:
C.M. Paquete
Author:
P.M. Pereira
Author:
T. Catarino
Author:
D.L. Turner
Author:
R.O. Louro
Author:
A.V. Xavier
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