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Collision-induced dissociation of three groups of hydroxamate siderophores: ferrioxamines, ferrichromes and coprogens/fusigens

Collision-induced dissociation of three groups of hydroxamate siderophores: ferrioxamines, ferrichromes and coprogens/fusigens
Collision-induced dissociation of three groups of hydroxamate siderophores: ferrioxamines, ferrichromes and coprogens/fusigens
The behaviour of a series of hydroxamate siderophores - microbially produced iron complexes - was investigated using electrospray ionisation mass spectrometry (ESI-MS). Three groups of iron hydroxamate siderophores, namely the ferrioxamines, ferrichromes and coprogens/fusigens, were separated by high-performance liquid chromatography (HPLC) prior to ESI and MS2 fragmentation. For the majority of the siderophores, both protonated molecules and sodium adducts were observed. The most abundant ion was selected for collision-induced fragmentation. Potential fragmentation mechanisms are postulated and discussed. Fragmentation patterns differed between siderophore groups; however, common fragmentation patterns were observed for siderophore ions within the groups examined. Cleavage frequently occurred at carbon-nitrogen or carbon-oxygen bonds. Fragmentation of the ions also involved cleavage of iron-oxygen bonds and transfer of the charge to iron.
0951-4198
2195-2202
Mawji, Edward
36970997-a479-4cc5-8120-82a2fe000202
Gledhill, Martha
da795c1e-1489-4d40-9df1-fc6bde54382d
Worsfold, Paul J.
27675f89-7eee-45c5-821e-a381d8db9693
Achterberg, Eric P.
685ce961-8c45-4503-9f03-50f6561202b9
Mawji, Edward
36970997-a479-4cc5-8120-82a2fe000202
Gledhill, Martha
da795c1e-1489-4d40-9df1-fc6bde54382d
Worsfold, Paul J.
27675f89-7eee-45c5-821e-a381d8db9693
Achterberg, Eric P.
685ce961-8c45-4503-9f03-50f6561202b9

Mawji, Edward, Gledhill, Martha, Worsfold, Paul J. and Achterberg, Eric P. (2008) Collision-induced dissociation of three groups of hydroxamate siderophores: ferrioxamines, ferrichromes and coprogens/fusigens. Rapid Communications in Mass Spectrometry, 22 (14), 2195-2202. (doi:10.1002/rcm.3604).

Record type: Article

Abstract

The behaviour of a series of hydroxamate siderophores - microbially produced iron complexes - was investigated using electrospray ionisation mass spectrometry (ESI-MS). Three groups of iron hydroxamate siderophores, namely the ferrioxamines, ferrichromes and coprogens/fusigens, were separated by high-performance liquid chromatography (HPLC) prior to ESI and MS2 fragmentation. For the majority of the siderophores, both protonated molecules and sodium adducts were observed. The most abundant ion was selected for collision-induced fragmentation. Potential fragmentation mechanisms are postulated and discussed. Fragmentation patterns differed between siderophore groups; however, common fragmentation patterns were observed for siderophore ions within the groups examined. Cleavage frequently occurred at carbon-nitrogen or carbon-oxygen bonds. Fragmentation of the ions also involved cleavage of iron-oxygen bonds and transfer of the charge to iron.

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Published date: 30 July 2008

Identifiers

Local EPrints ID: 54663
URI: http://eprints.soton.ac.uk/id/eprint/54663
ISSN: 0951-4198
PURE UUID: f863492f-562f-490c-b154-042d00da11b0

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Date deposited: 17 Jul 2008
Last modified: 15 Mar 2024 10:49

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Contributors

Author: Edward Mawji
Author: Martha Gledhill
Author: Paul J. Worsfold

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