The University of Southampton
University of Southampton Institutional Repository

Histidine-stimulated divalent metal uptake in human erythrocytes and in the erythroleukaemic cell line HEL.92.1.7

Oakley, F., Horn, N.M. and Thomas, A.L. (2004) Histidine-stimulated divalent metal uptake in human erythrocytes and in the erythroleukaemic cell line HEL.92.1.7 The Journal of Physiology, 561, (561), pp. 525-534. (doi:10.1113/jphysiol.2004.072389).

Record type: Article


The uptake of Zn-65 by human erythrocytes was investigated in the presence of high (40 mm) and low (5 mm) concentrations of histidine and 0-500 muM cobalt, nickel, manganese and zinc. Varying concentrations of metal mono- and bis-histidine complexes will be formed and the inhibition of Zn-65 uptake could be correlated with the calculated complex concentrations to investigate competition between metals. For each metal, the calculated concentrations of bis-histidine complex giving 50% inhibition of Zn-65 uptake were similar at both 5 mm and 40 mm histidine. Manganese-bis-histidine appeared to have a much higher affinity for the binding site than the other metal-bis-histidine complexes, which had similar affinities to each other. Studies of the inhibition of histidine-stimulated Mn-54 uptake by the addition of manganese confirmed that manganese-bis-histidine does act as a substrate for the transporter in a similar fashion to the other metals studied. In addition, human erythroleukaemic cells (HEL cells) were used as a model for erythroid precursor cells. L-histidine, but not D-histidine, stimulated Zn-65 uptake in a saturable fashion. The other metals competed with zinc in a similar manner to that seen in erythrocytes, and the affinity for manganese-bis-histidine was much greater than for the bis-histidine complexes of the other three metals. Both the capacity for metal transport per cell, and the affinity of the transporter for the metal-bis-histidine complexes, were much greater in the HEL cells than in the erythrocyte. It is suggested that histidine-stimulated metal transport may play a role in the supply of metals to maturing erythroid cells.

Full text not available from this repository.

More information

Submitted date: 22 July 2004
Published date: 14 October 2004
Organisations: Biological Sciences


Local EPrints ID: 55739
ISSN: 0022-3751
PURE UUID: 6ddd9a67-75d3-42d6-8548-50b08d6b82b9

Catalogue record

Date deposited: 05 Aug 2008
Last modified: 17 Jul 2017 14:32

Export record



Author: F. Oakley
Author: N.M. Horn
Author: A.L. Thomas

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.