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Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos

Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos
Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos
A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
human coagulation factor VII, transgenic fish, bioreactor
1436-2228
485-492
Hwang, Gyulin
f4b0347f-a35b-4e0c-938f-be2e5b40cb54
Muller, Ferenc
ed53409b-cf2f-4912-9aab-801ab44d06e2
Rahman, M. Aziz
ff49e5d4-4aed-41ca-ad44-ccf80d60db91
Williams, Darren W.
604e70da-4237-4069-87b0-6dff0917ca49
Murdock, Paul J.
6e328c24-1d75-4103-b9ec-edb948de685c
Pasi, K. John
3b81e0be-6e79-4b71-95c5-45fe04b8e6dc
Goldspink, Geoffrey
ced459a6-864c-48e3-b249-4e03edd6365c
Farahmand, Hamid
142f0cdf-014c-4335-abb3-95d807b7ea24
Maclean, Norman
cb0429bd-9dbc-48e7-b5b5-f429b63e6ad8
Hwang, Gyulin
f4b0347f-a35b-4e0c-938f-be2e5b40cb54
Muller, Ferenc
ed53409b-cf2f-4912-9aab-801ab44d06e2
Rahman, M. Aziz
ff49e5d4-4aed-41ca-ad44-ccf80d60db91
Williams, Darren W.
604e70da-4237-4069-87b0-6dff0917ca49
Murdock, Paul J.
6e328c24-1d75-4103-b9ec-edb948de685c
Pasi, K. John
3b81e0be-6e79-4b71-95c5-45fe04b8e6dc
Goldspink, Geoffrey
ced459a6-864c-48e3-b249-4e03edd6365c
Farahmand, Hamid
142f0cdf-014c-4335-abb3-95d807b7ea24
Maclean, Norman
cb0429bd-9dbc-48e7-b5b5-f429b63e6ad8

Hwang, Gyulin, Muller, Ferenc, Rahman, M. Aziz, Williams, Darren W., Murdock, Paul J., Pasi, K. John, Goldspink, Geoffrey, Farahmand, Hamid and Maclean, Norman (2004) Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos. Marine Biotechnology, 6 (5), 485-492. (doi:10.1007/s10126-004-3121-2).

Record type: Article

Abstract

A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.

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More information

Submitted date: 10 November 2003
Published date: September 2004
Keywords: human coagulation factor VII, transgenic fish, bioreactor

Identifiers

Local EPrints ID: 55779
URI: http://eprints.soton.ac.uk/id/eprint/55779
ISSN: 1436-2228
PURE UUID: a8869c30-9141-4829-8072-3d451e44a641

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Date deposited: 05 Aug 2008
Last modified: 07 Jan 2022 22:32

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Contributors

Author: Gyulin Hwang
Author: Ferenc Muller
Author: M. Aziz Rahman
Author: Darren W. Williams
Author: Paul J. Murdock
Author: K. John Pasi
Author: Geoffrey Goldspink
Author: Hamid Farahmand
Author: Norman Maclean

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