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Transgenic tilapia and the tilapia genome

Transgenic tilapia and the tilapia genome
Transgenic tilapia and the tilapia genome
The tilapia fish (Oreochromis niloticus) has an important place in the aquaculture of the developing world. It is also a very useful laboratory animal, and readily lends itself to the transgenic technology. Through the use of reporter genes, a range of potential gene promoters have been tested in tilapia, both through transient and stable expression of the reporter construct. Using the transgenic technology, growth enhanced lines of tilapia have been produced. These fish have no abnormalities and offer a considerable growth advantage for future exploitation. It is however crucial that transgenic fish, to be exploited in aquaculture, be sterile, and various methods of achieving sterility are considered. These include triploidy, gene knock out of crucial hormone encoding genes via homologous recombination, and knock down of the function of the same genes via ribozyme or antisense technologies.
transgenic fish, reporter gene, antisense, ribozyme, biofactory
0378-1119
265-277
Maclean, N.
fc7272ea-27ce-4f19-bb5b-dbccb8c520a8
Rahman, M.A.
77447cf8-c98d-478f-8d43-74bfc928cd11
Sohm, F.
7404941a-1e2f-4523-a894-70c73591c72a
Hwang, G.
4c75e280-de4d-4b51-b5a4-a91d87c87a91
Lyengar, A.
86ec59e3-8286-4493-8622-875c95e26c3a
Ayad, H.
59beb41d-1874-48ae-86a3-4afdb5e318aa
Smith, A.
f115f8cb-6c76-444b-ba80-7a1d54276da8
Farahmand, H.
29cff04a-f2b0-47cb-b535-82f79d7c3cfd
Maclean, N.
fc7272ea-27ce-4f19-bb5b-dbccb8c520a8
Rahman, M.A.
77447cf8-c98d-478f-8d43-74bfc928cd11
Sohm, F.
7404941a-1e2f-4523-a894-70c73591c72a
Hwang, G.
4c75e280-de4d-4b51-b5a4-a91d87c87a91
Lyengar, A.
86ec59e3-8286-4493-8622-875c95e26c3a
Ayad, H.
59beb41d-1874-48ae-86a3-4afdb5e318aa
Smith, A.
f115f8cb-6c76-444b-ba80-7a1d54276da8
Farahmand, H.
29cff04a-f2b0-47cb-b535-82f79d7c3cfd

Maclean, N., Rahman, M.A., Sohm, F., Hwang, G., Lyengar, A., Ayad, H., Smith, A. and Farahmand, H. (2002) Transgenic tilapia and the tilapia genome. Gene, 295 (2), 265-277. (doi:10.1016/S0378-1119(02)00735-7).

Record type: Article

Abstract

The tilapia fish (Oreochromis niloticus) has an important place in the aquaculture of the developing world. It is also a very useful laboratory animal, and readily lends itself to the transgenic technology. Through the use of reporter genes, a range of potential gene promoters have been tested in tilapia, both through transient and stable expression of the reporter construct. Using the transgenic technology, growth enhanced lines of tilapia have been produced. These fish have no abnormalities and offer a considerable growth advantage for future exploitation. It is however crucial that transgenic fish, to be exploited in aquaculture, be sterile, and various methods of achieving sterility are considered. These include triploidy, gene knock out of crucial hormone encoding genes via homologous recombination, and knock down of the function of the same genes via ribozyme or antisense technologies.

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More information

Submitted date: 10 August 2001
Published date: 7 August 2002
Keywords: transgenic fish, reporter gene, antisense, ribozyme, biofactory

Identifiers

Local EPrints ID: 55813
URI: http://eprints.soton.ac.uk/id/eprint/55813
ISSN: 0378-1119
PURE UUID: dff385c7-7061-49ab-8f80-b0bcc11ffcab

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Date deposited: 06 Aug 2008
Last modified: 15 Mar 2024 10:57

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Contributors

Author: N. Maclean
Author: M.A. Rahman
Author: F. Sohm
Author: G. Hwang
Author: A. Lyengar
Author: H. Ayad
Author: A. Smith
Author: H. Farahmand

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