Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene
Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene
We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also isolated two L18 cDNAs from another tilapia (Oreochromis niloticus) with a few conservative nucleotide differences. Our results suggest the presence of two genes in both species. Reporter constructs were tested for transient expression in CV1 cells and in microinjected zebrafish and tilapia embryos. The tilapia L18 promoter was able to drive expression of the reporter gene in all three experiments, with no apparent preference for a particular tissue. The tilapia L18 promoter is therefore likely to be a powerful tool to drive tissue-independent gene expression in fish.
ibosomal proteins, promoter, transcription, L18, fish, tilapia
195-202
Molina, Alfredo.
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Lyengar, Arati.
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Marins, Luis.F.
a818dd11-96fa-4f24-9316-2b9e459aea9b
Biemar, Frédéric
7d81cb07-a272-441b-bf15-fbff29ca9b7b
Hanley, Sean
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Maclean, Norman
cb0429bd-9dbc-48e7-b5b5-f429b63e6ad8
Smith, Terry.J.
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Martial, Joseph.A.
a3b081a3-8754-4a8c-9996-0f27ab43fb87
Muller, Marc
b23ef7a0-0958-486f-917a-84fd406184ee
21 September 2001
Molina, Alfredo.
03d97453-b408-4e79-a25e-17b5f1e27524
Lyengar, Arati.
989b79ca-5b2a-40eb-9cb2-9ce0a9c10cf6
Marins, Luis.F.
a818dd11-96fa-4f24-9316-2b9e459aea9b
Biemar, Frédéric
7d81cb07-a272-441b-bf15-fbff29ca9b7b
Hanley, Sean
a6fcfc8f-1e26-4598-aa2e-a506686f60b8
Maclean, Norman
cb0429bd-9dbc-48e7-b5b5-f429b63e6ad8
Smith, Terry.J.
4790bbf2-1574-43e9-977a-4c52b868b059
Martial, Joseph.A.
a3b081a3-8754-4a8c-9996-0f27ab43fb87
Muller, Marc
b23ef7a0-0958-486f-917a-84fd406184ee
Molina, Alfredo., Lyengar, Arati., Marins, Luis.F., Biemar, Frédéric, Hanley, Sean, Maclean, Norman, Smith, Terry.J., Martial, Joseph.A. and Muller, Marc
(2001)
Gene structure and promoter function of a teleost ribosomal protein: a tilapia (Oreochromis mossambicus) L18 gene.
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1520 (3), .
(doi:10.1016/S0167-4781(01)00272-X).
Abstract
We have cloned and characterized a tilapia (Oreochromis mossambicus) L18 ribosomal protein gene, including the complete transcribed region and 488 bp of upstream regulatory sequences. We have also isolated two L18 cDNAs from another tilapia (Oreochromis niloticus) with a few conservative nucleotide differences. Our results suggest the presence of two genes in both species. Reporter constructs were tested for transient expression in CV1 cells and in microinjected zebrafish and tilapia embryos. The tilapia L18 promoter was able to drive expression of the reporter gene in all three experiments, with no apparent preference for a particular tissue. The tilapia L18 promoter is therefore likely to be a powerful tool to drive tissue-independent gene expression in fish.
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More information
Submitted date: 2 January 2001
Published date: 21 September 2001
Keywords:
ibosomal proteins, promoter, transcription, L18, fish, tilapia
Identifiers
Local EPrints ID: 55815
URI: http://eprints.soton.ac.uk/id/eprint/55815
ISSN: 0167-4781
PURE UUID: 0654d0d0-4490-4090-9d69-4ac211952351
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Date deposited: 06 Aug 2008
Last modified: 15 Mar 2024 10:57
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Contributors
Author:
Alfredo. Molina
Author:
Arati. Lyengar
Author:
Luis.F. Marins
Author:
Frédéric Biemar
Author:
Sean Hanley
Author:
Norman Maclean
Author:
Terry.J. Smith
Author:
Joseph.A. Martial
Author:
Marc Muller
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