Confocal quality imaging of afferent neurons from semi-thin sections of Drosophila ganglia
Confocal quality imaging of afferent neurons from semi-thin sections of Drosophila ganglia
The aim of this study was to develop protocols for computer imaging of the thoraco-abdominal ganglion of Drosophila from serial semi-thin sections, in which specific neurons were stained and related to neuropilar structures. The central projections of a subset of transgenically labelled sensory neurons were revealed by immunohistochemistry, while Nomarski optics were used to show motor neuron targets in the neuropil. Digital photomicrographs of each section were aligned and the resultant image stacks rendered into three-dimensional (3D) images that can be rotated in real time. The result is a detailed, in-depth visualization of labelled neurons at a resolution comparable with that in confocal reconstructions, which also allows investigation of their relationships with other components of the neuropil.
drosophila, enhancer trap, computer imaging, three-dimensional reconstruction, monosynaptic connections
93-96
Tyrer, N. Mark
f90ab83e-020a-426a-a532-6d64461224c2
Maddison, John
a8a56af6-37f7-49cf-bb88-4f0eed00e76d
Shepherd, David
11aa6858-d19c-4450-82ff-11dff9dcd9c4
Williams, Darren W.
604e70da-4237-4069-87b0-6dff0917ca49
22 December 2000
Tyrer, N. Mark
f90ab83e-020a-426a-a532-6d64461224c2
Maddison, John
a8a56af6-37f7-49cf-bb88-4f0eed00e76d
Shepherd, David
11aa6858-d19c-4450-82ff-11dff9dcd9c4
Williams, Darren W.
604e70da-4237-4069-87b0-6dff0917ca49
Tyrer, N. Mark, Maddison, John, Shepherd, David and Williams, Darren W.
(2000)
Confocal quality imaging of afferent neurons from semi-thin sections of Drosophila ganglia.
Neuroscience Letters, 296 (2-3), .
(doi:10.1016/S0304-3940(00)01611-6).
Abstract
The aim of this study was to develop protocols for computer imaging of the thoraco-abdominal ganglion of Drosophila from serial semi-thin sections, in which specific neurons were stained and related to neuropilar structures. The central projections of a subset of transgenically labelled sensory neurons were revealed by immunohistochemistry, while Nomarski optics were used to show motor neuron targets in the neuropil. Digital photomicrographs of each section were aligned and the resultant image stacks rendered into three-dimensional (3D) images that can be rotated in real time. The result is a detailed, in-depth visualization of labelled neurons at a resolution comparable with that in confocal reconstructions, which also allows investigation of their relationships with other components of the neuropil.
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Published date: 22 December 2000
Keywords:
drosophila, enhancer trap, computer imaging, three-dimensional reconstruction, monosynaptic connections
Identifiers
Local EPrints ID: 55849
URI: http://eprints.soton.ac.uk/id/eprint/55849
ISSN: 0304-3940
PURE UUID: a4d33495-a983-4c47-bef8-bf2193a03c5f
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Date deposited: 21 Aug 2008
Last modified: 15 Mar 2024 10:58
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Author:
N. Mark Tyrer
Author:
John Maddison
Author:
Darren W. Williams
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