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Quantitative proteomics identifies Gemin5, a scaffolding protein involved in ribonucleoprotein assembly, as a novel partner for eukaryotic initiation factor 4E

Fierro-Monti, I., Mohammed, S., Matthiesen, R., Santoro, R., Burns, J.S., Williams, D.J., Proud, C.G., Kassem, M., Jensen, O.N. and Roepstorff, P. (2006) Quantitative proteomics identifies Gemin5, a scaffolding protein involved in ribonucleoprotein assembly, as a novel partner for eukaryotic initiation factor 4E Journal of Proteome Research, 5, (6), pp. 1367-1378. (doi:10.1021/pr0504539).

Record type: Article


Protein complexes are dynamic entities; identification and quantitation of their components is critical in elucidating functional roles under specific cellular conditions. We report the first quantitative proteomic analysis of the human cap-binding protein complex. Components and proteins associated with the translation initiation eIF4F complex that may affect complex formation were identified and quantitated under distinct growth conditions. Site-specific phosphorylation of eIF4E and eIF4G and elevated levels of eIF4G:eIF4E complexes in phorbol ester treated HEK293 cells, and in serum-starved tumorigenic human mesenchymal stromal cells, attested to their activated translational states. The WD-repeat, scaffolding-protein Gemin5 was identified as a novel eIF4E binding partner, which interacted directly with eIF4E through a motif (YXXXXL) present in a number of eIF4E-interacting partners. Elevated levels of Gemin5:eIF4E complexes were found in phorbol ester treated HEK293 cells. Gemin5 and eIF4E co-localized to cytoplasmic P-bodies in human osteosarcoma U2OS cells. Interaction between eIF4E and Gemin5 and their co-localization to the P-bodies, may serve to recruit capped mRNAs to these RNP complexes, for functions related to RNP assembly, remodeling and/or transition from active translation to mRNA degradation. Our results demonstrate that our quantitative proteomic strategy can be applied to the identification and quantitation of protein complex components in human cells grown under different conditions.

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Published date: 1 June 2006
Keywords: quantitative mass spectrometry, isotope labeling, translation initiation complex, eIF4E interaction, eIF4E phosphorylation, eIF4G phosphorylation, Gemin5 interaction, processing bodies


Local EPrints ID: 55852
ISSN: 1535-3893
PURE UUID: 5277c689-daa9-4d64-b3d1-9d0cc10c408c

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Date deposited: 06 Aug 2008
Last modified: 17 Jul 2017 14:31

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Author: I. Fierro-Monti
Author: S. Mohammed
Author: R. Matthiesen
Author: R. Santoro
Author: J.S. Burns
Author: D.J. Williams
Author: C.G. Proud
Author: M. Kassem
Author: O.N. Jensen
Author: P. Roepstorff

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