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Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes

Record type: Article

We have isolated the novel gene SMOC-1 that encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain. Recombinant expression in human cells showed that SMOC-1 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots, reverse transcriptase-PCR, and immunoblots revealed a widespread expression in many tissues. Immunofluorescence studies with an antiserum directed against recombinant human SMOC-1 demonstrated a basement membrane localization of the protein and additionally its presence in other extracellular matrices. Immunogold electron microscopy confirmed the localization of SMOC-1 within basement membranes in kidney and skeletal muscle as well as its expression in the zona pellucida surrounding the oocyte.

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Citation

Vannahme, C., Smyth, N., Miosge, N., Gosling, S., Frie, C., Paulsson, M., Maurer, P. and Hartmann, U. (2002) Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes The Journal of Biological Chemistry, 277, (41), pp. 37977-37986. (doi:10.1074/jbc.M203830200).

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Published date: 1 October 2002

Identifiers

Local EPrints ID: 55856
URI: http://eprints.soton.ac.uk/id/eprint/55856
ISSN: 0021-9258
PURE UUID: bc9b2b5c-d7ec-4dcb-acfa-e7d7c5cd608f

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Date deposited: 06 Aug 2008
Last modified: 17 Jul 2017 14:31

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Contributors

Author: C. Vannahme
Author: N. Smyth
Author: N. Miosge
Author: S. Gosling
Author: C. Frie
Author: M. Paulsson
Author: P. Maurer
Author: U. Hartmann

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