Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes


Vannahme, C., Smyth, N., Miosge, N., Gosling, S., Frie, C., Paulsson, M., Maurer, P. and Hartmann, U. (2002) Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes The Journal of Biological Chemistry, 277, (41), pp. 37977-37986. (doi:10.1074/jbc.M203830200).

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Description/Abstract

We have isolated the novel gene SMOC-1 that encodes a secreted modular protein containing an EF-hand calcium-binding domain homologous to that in BM-40. It further consists of two thyroglobulin-like domains, a follistatin-like domain and a novel domain. Recombinant expression in human cells showed that SMOC-1 is a glycoprotein with a calcium-dependent conformation. Results from Northern blots, reverse transcriptase-PCR, and immunoblots revealed a widespread expression in many tissues. Immunofluorescence studies with an antiserum directed against recombinant human SMOC-1 demonstrated a basement membrane localization of the protein and additionally its presence in other extracellular matrices. Immunogold electron microscopy confirmed the localization of SMOC-1 within basement membranes in kidney and skeletal muscle as well as its expression in the zona pellucida surrounding the oocyte.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1074/jbc.M203830200
ISSNs: 0021-9258 (print)
Subjects:
ePrint ID: 55856
Date :
Date Event
1 October 2002Published
Date Deposited: 06 Aug 2008
Last Modified: 16 Apr 2017 17:42
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/55856

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