Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue]
Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue]
Background: There is evidence in humans and experimental
animals for non-genomic transmission of induced phenotypes
between generations. Induction of an altered phenotype in
the F1 generation by feeding a protein-restricted (PR) diet to
pregnant rats involves altered epigenetic regulation of
specific genes. We investigated whether altered epigenetic
regulation of hepatic PPARa and glucocorticoid receptor (GR)
promoters induced in the F1 generation by prenatal undernutrition
is passed to the F2 offspring.
Methods: Rats (F0) were fed a control (18% protein) or PR
(9% protein) diet throughout pregnancy and chow during
lactation. F1 females were mated and fed chow throughout
pregnancy and lactation. F1 and F2 offspring were fed chow.
Male offspring from the F1 and F2 generations (n =6 per F0
dietary group) were killed at day 80. Methylation of the
hepatic PPARa and GR promoters was determined by
methylation-sensitive real-time PCR. mRNA expression was
measured by real-time RT-PCR.
Results: Methylation of the hepatic PPARa and GR promoters
was lower (8% to 11%, p b0.05) in the F1 and F2 PR offspring.
There were trends towards higher PPARa and GR expression
in the PR offspring.
p.495
Burdge, G.C.
bbf0edb0-d7e2-4e0b-8ca8-a9710f9a1fca
Slater-Jefferies, J.L.
e46c711a-9d4c-436a-b853-828df69bb4d7
Torrens, C.
15a35713-0651-4249-8227-5901e2cfcd22
Phillips, E.S.
66ddd4cb-19a2-4d08-889b-12f418e6878b
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lillycrop, K.A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
1 August 2006
Burdge, G.C.
bbf0edb0-d7e2-4e0b-8ca8-a9710f9a1fca
Slater-Jefferies, J.L.
e46c711a-9d4c-436a-b853-828df69bb4d7
Torrens, C.
15a35713-0651-4249-8227-5901e2cfcd22
Phillips, E.S.
66ddd4cb-19a2-4d08-889b-12f418e6878b
Hanson, M.A.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Lillycrop, K.A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Burdge, G.C., Slater-Jefferies, J.L., Torrens, C., Phillips, E.S., Hanson, M.A. and Lillycrop, K.A.
(2006)
Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue].
Early Human Development, 82 (8), .
Abstract
Background: There is evidence in humans and experimental
animals for non-genomic transmission of induced phenotypes
between generations. Induction of an altered phenotype in
the F1 generation by feeding a protein-restricted (PR) diet to
pregnant rats involves altered epigenetic regulation of
specific genes. We investigated whether altered epigenetic
regulation of hepatic PPARa and glucocorticoid receptor (GR)
promoters induced in the F1 generation by prenatal undernutrition
is passed to the F2 offspring.
Methods: Rats (F0) were fed a control (18% protein) or PR
(9% protein) diet throughout pregnancy and chow during
lactation. F1 females were mated and fed chow throughout
pregnancy and lactation. F1 and F2 offspring were fed chow.
Male offspring from the F1 and F2 generations (n =6 per F0
dietary group) were killed at day 80. Methylation of the
hepatic PPARa and GR promoters was determined by
methylation-sensitive real-time PCR. mRNA expression was
measured by real-time RT-PCR.
Results: Methylation of the hepatic PPARa and GR promoters
was lower (8% to 11%, p b0.05) in the F1 and F2 PR offspring.
There were trends towards higher PPARa and GR expression
in the PR offspring.
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Published date: 1 August 2006
Additional Information:
Abstracts of the 4th World Congress on Developmental Origins of Health and Disease (DOHaD), 13-16 September 2006, Educatorium, University of Utrecht, The Netherlands
Organisations:
Biological Sciences, Medicine
Identifiers
Local EPrints ID: 55884
URI: http://eprints.soton.ac.uk/id/eprint/55884
ISSN: 0378-3782
PURE UUID: 545f11d5-ba27-4841-bbaf-cff3d7aeb91b
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Date deposited: 06 Aug 2008
Last modified: 16 Mar 2024 03:50
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Contributors
Author:
G.C. Burdge
Author:
C. Torrens
Author:
E.S. Phillips
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