The University of Southampton
University of Southampton Institutional Repository

Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue]

Record type: Article

Background: There is evidence in humans and experimental animals for non-genomic transmission of induced phenotypes between generations. Induction of an altered phenotype in the F1 generation by feeding a protein-restricted (PR) diet to pregnant rats involves altered epigenetic regulation of specific genes. We investigated whether altered epigenetic regulation of hepatic PPARa and glucocorticoid receptor (GR) promoters induced in the F1 generation by prenatal undernutrition is passed to the F2 offspring.
Methods: Rats (F0) were fed a control (18% protein) or PR (9% protein) diet throughout pregnancy and chow during lactation. F1 females were mated and fed chow throughout pregnancy and lactation. F1 and F2 offspring were fed chow. Male offspring from the F1 and F2 generations (n =6 per F0 dietary group) were killed at day 80. Methylation of the hepatic PPARa and GR promoters was determined by methylation-sensitive real-time PCR. mRNA expression was measured by real-time RT-PCR.
Results: Methylation of the hepatic PPARa and GR promoters was lower (8% to 11%, p b0.05) in the F1 and F2 PR offspring. There were trends towards higher PPARa and GR expression in the PR offspring.

Full text not available from this repository.

Citation

Burdge, G.C., Slater-Jefferies, J.L., Torrens, C., Phillips, E.S., Hanson, M.A. and Lillycrop, K.A. (2006) Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue] Early Human Development, 82, (8), p.495. (doi:10.1016/j.earlhumdev.2006.06.003).

More information

Published date: 1 August 2006
Additional Information: Abstracts of the 4th World Congress on Developmental Origins of Health and Disease (DOHaD), 13-16 September 2006, Educatorium, University of Utrecht, The Netherlands
Organisations: Biological Sciences, Medicine

Identifiers

Local EPrints ID: 55884
URI: http://eprints.soton.ac.uk/id/eprint/55884
ISSN: 0378-3782
PURE UUID: 545f11d5-ba27-4841-bbaf-cff3d7aeb91b
ORCID for E.S. Phillips: ORCID iD orcid.org/0000-0001-5268-4203
ORCID for K.A. Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 17 Jul 2017 14:31

Export record

Altmetrics

Contributors

Author: G.C. Burdge
Author: J.L. Slater-Jefferies
Author: C. Torrens
Author: E.S. Phillips ORCID iD
Author: M.A. Hanson
Author: K.A. Lillycrop ORCID iD

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×