Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue]


Burdge, G.C., Slater-Jefferies, J.L., Torrens, C., Phillips, E.S., Hanson, M.A. and Lillycrop, K.A. (2006) Feeding a protein-restricted diet during pregnancy in the rat induces altered gene promoter methylation in the liver of the F1 and F2 offspring [In special abstracts issue] Early Human Development, 82, (8), p.495. (doi:10.1016/j.earlhumdev.2006.06.003).

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Description/Abstract

Background: There is evidence in humans and experimental animals for non-genomic transmission of induced phenotypes between generations. Induction of an altered phenotype in the F1 generation by feeding a protein-restricted (PR) diet to pregnant rats involves altered epigenetic regulation of specific genes. We investigated whether altered epigenetic regulation of hepatic PPARa and glucocorticoid receptor (GR) promoters induced in the F1 generation by prenatal undernutrition is passed to the F2 offspring.
Methods: Rats (F0) were fed a control (18% protein) or PR (9% protein) diet throughout pregnancy and chow during lactation. F1 females were mated and fed chow throughout pregnancy and lactation. F1 and F2 offspring were fed chow. Male offspring from the F1 and F2 generations (n =6 per F0 dietary group) were killed at day 80. Methylation of the hepatic PPARa and GR promoters was determined by methylation-sensitive real-time PCR. mRNA expression was measured by real-time RT-PCR.
Results: Methylation of the hepatic PPARa and GR promoters was lower (8% to 11%, p b0.05) in the F1 and F2 PR offspring. There were trends towards higher PPARa and GR expression in the PR offspring.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.earlhumdev.2006.06.003
Additional Information: Abstracts of the 4th World Congress on Developmental Origins of Health and Disease (DOHaD), 13-16 September 2006, Educatorium, University of Utrecht, The Netherlands
ISSNs: 0378-3782 (print)
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ePrint ID: 55884
Date :
Date Event
1 August 2006Published
Date Deposited: 06 Aug 2008
Last Modified: 16 Apr 2017 17:42
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/55884

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