The University of Southampton
University of Southampton Institutional Repository

Inosine substitutions demonstrate that intramolecular DNA quadruplexes adopt different conformations in the presence of sodium and potassium

Inosine substitutions demonstrate that intramolecular DNA quadruplexes adopt different conformations in the presence of sodium and potassium
Inosine substitutions demonstrate that intramolecular DNA quadruplexes adopt different conformations in the presence of sodium and potassium
We have examined the stability of fluorescently-labelled oligonucleotides that are based on the human telomeric repeat [(GGGTTA)3GGG], in which one of the guanines in turn is substituted with inosine. We show that the relative stability of the substitutions is different in the presence of sodium and potassium. The data for potassium suggest a parallel arrangement of the strands, while the sodium form is mixed parallel and antiparallel.
Graphical abstract The relative stability of intramolecular quadruplexes containing inosine substitutions is different in sodium and potassium.
G-quartet, quadruplex, fluorescence, molecular beacon, DNA melting, inosine
2047-2050
Risitano, A.
cf59ac40-0ade-4e18-ab7e-354b4026b8a3
Fox, K.R.
9da5debc-4e45-473e-ab8c-550d1104659f
Risitano, A.
cf59ac40-0ade-4e18-ab7e-354b4026b8a3
Fox, K.R.
9da5debc-4e45-473e-ab8c-550d1104659f

Risitano, A. and Fox, K.R. (2005) Inosine substitutions demonstrate that intramolecular DNA quadruplexes adopt different conformations in the presence of sodium and potassium. Bioorganic & Medicinal Chemistry Letters, 15 (8), 2047-2050. (doi:10.1016/j.bmcl.2005.02.050).

Record type: Article

Abstract

We have examined the stability of fluorescently-labelled oligonucleotides that are based on the human telomeric repeat [(GGGTTA)3GGG], in which one of the guanines in turn is substituted with inosine. We show that the relative stability of the substitutions is different in the presence of sodium and potassium. The data for potassium suggest a parallel arrangement of the strands, while the sodium form is mixed parallel and antiparallel.
Graphical abstract The relative stability of intramolecular quadruplexes containing inosine substitutions is different in sodium and potassium.

Full text not available from this repository.

More information

Published date: 15 April 2005
Keywords: G-quartet, quadruplex, fluorescence, molecular beacon, DNA melting, inosine

Identifiers

Local EPrints ID: 55887
URI: https://eprints.soton.ac.uk/id/eprint/55887
PURE UUID: 6a1cc652-04e3-4201-8c22-abbc06f351ac
ORCID for K.R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 24 Oct 2018 00:37

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×