SH3-SH2 domain orientation in Src kinases: NMR studies of Fyn
SH3-SH2 domain orientation in Src kinases: NMR studies of Fyn
The regulatory domains of Src family kinases SH3 and SH2 suppress Src activity when bound to the catalytic domain. Here, the isolated SH3-SH2 fragment from the Src family member Fyn (FynSH32) is studied by NMR. The properties of this fragment are expected to be similar to the domains in the active state, where they are dissociated from the catalytic domain. Crosscommunication between SH3 and SH2 of FynSH32, measured by chemical shift perturbation, was found to be small. Diffusion and alignment anisotropy measurements showed that SH3 and SH2 of peptide-bound FynSH32 are significantly coupled but still exhibit some interdomain flexibility. The observed average domain orientation indicates that a large SH3-SH2 domain closure is required to reach the inactive state. The implications of these results for Src regulation are discussed.
901-911
Ulmer, T.S.
6a7fbd4d-5a48-4945-9f58-ff79336bfd85
Werner, J.M.
1b02513a-8310-4f4f-adac-dc2a466bd115
Campbell, I.D.
36f9eac7-5354-4334-9d77-dc660f260ef1
1 July 2002
Ulmer, T.S.
6a7fbd4d-5a48-4945-9f58-ff79336bfd85
Werner, J.M.
1b02513a-8310-4f4f-adac-dc2a466bd115
Campbell, I.D.
36f9eac7-5354-4334-9d77-dc660f260ef1
Ulmer, T.S., Werner, J.M. and Campbell, I.D.
(2002)
SH3-SH2 domain orientation in Src kinases: NMR studies of Fyn.
Structure, 10 (Jul), .
Abstract
The regulatory domains of Src family kinases SH3 and SH2 suppress Src activity when bound to the catalytic domain. Here, the isolated SH3-SH2 fragment from the Src family member Fyn (FynSH32) is studied by NMR. The properties of this fragment are expected to be similar to the domains in the active state, where they are dissociated from the catalytic domain. Crosscommunication between SH3 and SH2 of FynSH32, measured by chemical shift perturbation, was found to be small. Diffusion and alignment anisotropy measurements showed that SH3 and SH2 of peptide-bound FynSH32 are significantly coupled but still exhibit some interdomain flexibility. The observed average domain orientation indicates that a large SH3-SH2 domain closure is required to reach the inactive state. The implications of these results for Src regulation are discussed.
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Published date: 1 July 2002
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Local EPrints ID: 55890
URI: http://eprints.soton.ac.uk/id/eprint/55890
ISSN: 0969-2126
PURE UUID: 12e227bc-b562-409c-8ac8-a14391dccdf5
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Date deposited: 06 Aug 2008
Last modified: 09 Jan 2022 03:14
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Author:
T.S. Ulmer
Author:
I.D. Campbell
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