Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H+-ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe cichoracearum

Fotopoulos, V., Holmes, R., Hall, J.L. and Williams, L.E. (2006) Isolation, cloning and expression analysis of EcPMA1, a putative plasma membrane H+-ATPase transporter gene from the biotrophic pathogenic fungus Erysiphe cichoracearum. Mycological Research, 110 (1), 28-37. (doi:10.1016/j.mycres.2005.08.011).

Record type: Article

Abstract

Little is known at the molecular level about the transporters involved in nutrient transfer in the plant/powdery mildew interaction. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for an isoform of the plasma membrane H+-ATPase (EcPMA1) in the biotrophic pathogenic fungus Erysiphe cichoracearum. Southern analysis suggests that EcPMA1 exists as a single-copy gene. Sequence analysis indicated a high similarity of EcPMA1 to other fungal H+-ATPases. Expression of EcPMA1 increases in infected Arabidopsis leaves as the disease progresses, correlating with the growth of the pathogen.

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