Nerve growth factor induces the expression of the LIM homeodomain transcription factor Isl-1 with the kinetics of an immediate early gene in adult rat dorsal root ganglion
Nerve growth factor induces the expression of the LIM homeodomain transcription factor Isl-1 with the kinetics of an immediate early gene in adult rat dorsal root ganglion
LIM-homeodomain genes encode a major class of transcription factors which play a critical role in regulating tissue specific gene expression. In this report, we have shown that three members of the LIM-homeodomain gene family - Isl-1, Rlim and Lim-3 are expressed in adult rat sensory neurons. Furthermore, we show that the addition of nerve growth factor (NGF) to cultures of primary dorsal root ganglion neurons leads to the induction of Isl-1, Rlim and Lim-3 mRNA expression. The increase in Isl-1 mRNA levels upon NGF addition was rapid and occurred even in the presence of cycloheximide. These findings place Isl-1 as a novel member of the immediate early class of genes. In contrast, Rlim and Lim-3mRNA induction by NGF required protein synthesis. The role of LIM-homeodomain genes in mediating responses to NGF in adult sensory neurons is discussed.
sensory neuron, dorsal root ganglion, LIM-homeodomain genes, nerve growth factor, immediate early genes, growth factors
130-134
Jameson, H.L.
17b069d2-f26f-401d-9cb0-7ccdca8eb390
Lillycrop, K.A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
24 August 2001
Jameson, H.L.
17b069d2-f26f-401d-9cb0-7ccdca8eb390
Lillycrop, K.A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Jameson, H.L. and Lillycrop, K.A.
(2001)
Nerve growth factor induces the expression of the LIM homeodomain transcription factor Isl-1 with the kinetics of an immediate early gene in adult rat dorsal root ganglion.
Neuroscience Letters, 309 (2), .
(doi:10.1016/S0304-3940(01)02040-7).
Abstract
LIM-homeodomain genes encode a major class of transcription factors which play a critical role in regulating tissue specific gene expression. In this report, we have shown that three members of the LIM-homeodomain gene family - Isl-1, Rlim and Lim-3 are expressed in adult rat sensory neurons. Furthermore, we show that the addition of nerve growth factor (NGF) to cultures of primary dorsal root ganglion neurons leads to the induction of Isl-1, Rlim and Lim-3 mRNA expression. The increase in Isl-1 mRNA levels upon NGF addition was rapid and occurred even in the presence of cycloheximide. These findings place Isl-1 as a novel member of the immediate early class of genes. In contrast, Rlim and Lim-3mRNA induction by NGF required protein synthesis. The role of LIM-homeodomain genes in mediating responses to NGF in adult sensory neurons is discussed.
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Published date: 24 August 2001
Keywords:
sensory neuron, dorsal root ganglion, LIM-homeodomain genes, nerve growth factor, immediate early genes, growth factors
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Local EPrints ID: 55901
URI: http://eprints.soton.ac.uk/id/eprint/55901
ISSN: 0304-3940
PURE UUID: f33dc0e8-bfa3-49c5-95c9-e7de54cf6c12
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Date deposited: 06 Aug 2008
Last modified: 16 Mar 2024 02:50
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Author:
H.L. Jameson
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