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Altered chemokine expression in the spinal cord and brain contributes to differential interleukin-1 beta-induced neutrophil recruitment

Altered chemokine expression in the spinal cord and brain contributes to differential interleukin-1 beta-induced neutrophil recruitment
Altered chemokine expression in the spinal cord and brain contributes to differential interleukin-1 beta-induced neutrophil recruitment
The pattern of neutrophil recruitment that accompanies inflammation in the CNS depends on the site of injury and the stage of development. The adult brain parenchyma is refractory to neutrophil recruitment and associated damage as compared to the spinal cord or juvenile brain. Using quantitative Taqman RT–PCR and enzyme-liked immunosorbent assay (ELISA), we compared mRNA and protein expression of the rat neutrophil chemoattractant chemokines (CINC) in spinal cord and brain of adult and juvenile rats to identify possible association with the observed differences in neutrophil recruitment. Interleukin-1? (IL-1?) injection resulted in up-regulated chemokine expression in both brain and spinal cord. CINC-3 mRNA was elevated above CINC-1 and CINC-2?, with expression levels for each higher in spinal cord than in brain. By ELISA, IL-1? induced greater CINC-1 and CINC-2? expression compared to CINC-3, with higher protein levels in spinal cord than in brain. In the juvenile brain, significantly higher levels of CINC-2? protein were observed in response to IL-1? injection than in the adult brain following an equivalent challenge. Correspondingly, neutrophil recruitment was observed in the juvenile brain and adult spinal cord, but not in the adult brain. No expression of CINC-2? mRNA was detected. Thus differential chemokine induction may contribute to variations in neutrophil recruitment in during development and between the different CNS compartments.
0022-3042
432-441
Campbell, Sandra J.
5c85ee6c-22f6-47d9-9e11-21173a9323a5
Wilcockson, David C.
ba84a5a7-c06c-4d54-ba4d-1113c607e28e
Butchart, Angus G.
0a43e963-c346-415a-b64f-30327c8cf1a5
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Anthony, Daniel C.
928249fa-dcf4-4088-b95b-ce14c719164d
Campbell, Sandra J.
5c85ee6c-22f6-47d9-9e11-21173a9323a5
Wilcockson, David C.
ba84a5a7-c06c-4d54-ba4d-1113c607e28e
Butchart, Angus G.
0a43e963-c346-415a-b64f-30327c8cf1a5
Perry, V. Hugh
8f29d36a-8e1f-4082-8700-09483bbaeae4
Anthony, Daniel C.
928249fa-dcf4-4088-b95b-ce14c719164d

Campbell, Sandra J., Wilcockson, David C., Butchart, Angus G., Perry, V. Hugh and Anthony, Daniel C. (2002) Altered chemokine expression in the spinal cord and brain contributes to differential interleukin-1 beta-induced neutrophil recruitment. Journal of Neurochemistry, 83 (2), 432-441. (doi:10.1046/j.1471-4159.2002.01166.x).

Record type: Article

Abstract

The pattern of neutrophil recruitment that accompanies inflammation in the CNS depends on the site of injury and the stage of development. The adult brain parenchyma is refractory to neutrophil recruitment and associated damage as compared to the spinal cord or juvenile brain. Using quantitative Taqman RT–PCR and enzyme-liked immunosorbent assay (ELISA), we compared mRNA and protein expression of the rat neutrophil chemoattractant chemokines (CINC) in spinal cord and brain of adult and juvenile rats to identify possible association with the observed differences in neutrophil recruitment. Interleukin-1? (IL-1?) injection resulted in up-regulated chemokine expression in both brain and spinal cord. CINC-3 mRNA was elevated above CINC-1 and CINC-2?, with expression levels for each higher in spinal cord than in brain. By ELISA, IL-1? induced greater CINC-1 and CINC-2? expression compared to CINC-3, with higher protein levels in spinal cord than in brain. In the juvenile brain, significantly higher levels of CINC-2? protein were observed in response to IL-1? injection than in the adult brain following an equivalent challenge. Correspondingly, neutrophil recruitment was observed in the juvenile brain and adult spinal cord, but not in the adult brain. No expression of CINC-2? mRNA was detected. Thus differential chemokine induction may contribute to variations in neutrophil recruitment in during development and between the different CNS compartments.

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Published date: 1 October 2002

Identifiers

Local EPrints ID: 55949
URI: http://eprints.soton.ac.uk/id/eprint/55949
ISSN: 0022-3042
PURE UUID: d19972e9-e6bb-40fc-abad-63503cde1ac6

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Date deposited: 07 Aug 2008
Last modified: 13 Mar 2019 20:35

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Contributors

Author: Sandra J. Campbell
Author: David C. Wilcockson
Author: Angus G. Butchart
Author: V. Hugh Perry
Author: Daniel C. Anthony

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