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Age-related increase in levels of insecticidal protein in the progenies of transgenic oilseed rape and its efficacy against a susceptible strain of diamondback moth

Age-related increase in levels of insecticidal protein in the progenies of transgenic oilseed rape and its efficacy against a susceptible strain of diamondback moth
Age-related increase in levels of insecticidal protein in the progenies of transgenic oilseed rape and its efficacy against a susceptible strain of diamondback moth
Many crops transformed with insecticidal genes isolated from Bacillus thuringiensis (Bt) show resistance to targeted insect pests. The concentration of Bt endotoxin proteins in plants is very important in transgenic crop efficacy and risk assessment. In the present study, changes in levels of CrylAc protein in the leaves of transgenic Bt oilseed rape (Brassica napus) carrying a Bt crylAc gene under the control of the cauliflower mosaic virus 35S promoter were quantified during vegetative growth by enzyme-linked immunosorbent assay. Plants were grown in a glasshouse, sampled at 2, 4, 5 and 6 weeks, and the concentration of CrylAc was quantified in basal, top and previous top leaves. The mean concentration differed between sowing dates when CrylAc concentration was expressed as ng g-1 fresh leaf weight but not when expressed as ng mg-1 total soluble protein. It was demonstrated that CrylAc concentration increased significantly as the leaf aged, while the total soluble plant protein decreased significantly. Levels of CrylAc were therefore higher in leaves at the base of the plants than in leaves close to the growing point. However, even young leaves with very low CrylAc concentrations caused high mortality in the larvae of a CrylAc-susceptible laboratory strain of the diamondback moth. The feeding area of leaves consumed by larvae in vivo and in situ was similar. Leaf damage caused by sampling (i.e. artificially) or by feeding of larvae did not affect the levels of CrylAc in the leaves under the experimental conditions in this study.
Biochemical compound, Entomopathogen, Bacteria, Bacillales, Bacillaceae, Bacillus thuringiensis, Pest, Genetically modified organism, Biotechnology, Genetic engineering, Vegetative apparatus, Phytophagous, Oil plant (vegetal), Invertebrata, Arthropoda, Insecta, Lepidoptera, Plutellidae, Spermatophyta, Angiospermae, Dicotyledones, Cruciferae, Biopesticide, Biology, Plant production, Genetic transfer, Protein, Plutella xylostella, Brassica napus var. oleifera, Transgenic plant, Gene expression, Assay, Plant leaf, Strain, Sensitivity resistance, Genetic transformation, Progeny, Microbial insecticide, Age
0003-4746
227-234
Wei, W.
c8964a0e-634a-408b-8d5c-c77238f3046d
Schuler, T.H.
f6edcf77-93e2-4e08-9167-96f17a0acf98
Clark, S.J.
671192a5-76fb-4fc1-a693-db782b74ea56
Stewart, C.N.
ed3b6e73-62b7-4a21-a202-58b6277c8e7c
Poppy, G.M.
e18524cf-10ae-4ab4-b50c-e73e7d841389
Wei, W.
c8964a0e-634a-408b-8d5c-c77238f3046d
Schuler, T.H.
f6edcf77-93e2-4e08-9167-96f17a0acf98
Clark, S.J.
671192a5-76fb-4fc1-a693-db782b74ea56
Stewart, C.N.
ed3b6e73-62b7-4a21-a202-58b6277c8e7c
Poppy, G.M.
e18524cf-10ae-4ab4-b50c-e73e7d841389

Wei, W., Schuler, T.H., Clark, S.J., Stewart, C.N. and Poppy, G.M. (2005) Age-related increase in levels of insecticidal protein in the progenies of transgenic oilseed rape and its efficacy against a susceptible strain of diamondback moth. Annals of Applied Biology, 147 (3), 227-234. (doi:10.1111/j.1744-7348.2005.00024.x).

Record type: Article

Abstract

Many crops transformed with insecticidal genes isolated from Bacillus thuringiensis (Bt) show resistance to targeted insect pests. The concentration of Bt endotoxin proteins in plants is very important in transgenic crop efficacy and risk assessment. In the present study, changes in levels of CrylAc protein in the leaves of transgenic Bt oilseed rape (Brassica napus) carrying a Bt crylAc gene under the control of the cauliflower mosaic virus 35S promoter were quantified during vegetative growth by enzyme-linked immunosorbent assay. Plants were grown in a glasshouse, sampled at 2, 4, 5 and 6 weeks, and the concentration of CrylAc was quantified in basal, top and previous top leaves. The mean concentration differed between sowing dates when CrylAc concentration was expressed as ng g-1 fresh leaf weight but not when expressed as ng mg-1 total soluble protein. It was demonstrated that CrylAc concentration increased significantly as the leaf aged, while the total soluble plant protein decreased significantly. Levels of CrylAc were therefore higher in leaves at the base of the plants than in leaves close to the growing point. However, even young leaves with very low CrylAc concentrations caused high mortality in the larvae of a CrylAc-susceptible laboratory strain of the diamondback moth. The feeding area of leaves consumed by larvae in vivo and in situ was similar. Leaf damage caused by sampling (i.e. artificially) or by feeding of larvae did not affect the levels of CrylAc in the leaves under the experimental conditions in this study.

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More information

Published date: 1 January 2005
Keywords: Biochemical compound, Entomopathogen, Bacteria, Bacillales, Bacillaceae, Bacillus thuringiensis, Pest, Genetically modified organism, Biotechnology, Genetic engineering, Vegetative apparatus, Phytophagous, Oil plant (vegetal), Invertebrata, Arthropoda, Insecta, Lepidoptera, Plutellidae, Spermatophyta, Angiospermae, Dicotyledones, Cruciferae, Biopesticide, Biology, Plant production, Genetic transfer, Protein, Plutella xylostella, Brassica napus var. oleifera, Transgenic plant, Gene expression, Assay, Plant leaf, Strain, Sensitivity resistance, Genetic transformation, Progeny, Microbial insecticide, Age

Identifiers

Local EPrints ID: 55958
URI: https://eprints.soton.ac.uk/id/eprint/55958
ISSN: 0003-4746
PURE UUID: 5e0025d8-6f01-450b-85b2-f0d7b8738e88

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Date deposited: 07 Aug 2008
Last modified: 13 Mar 2019 20:35

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Contributors

Author: W. Wei
Author: T.H. Schuler
Author: S.J. Clark
Author: C.N. Stewart
Author: G.M. Poppy

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