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Brush border structure defects in I-plastin deficient mice

Grimm-Günter, Eva-Maria S., Ramos, Sonia, Smyth, Neil, Celine, Revenu, Hurbain, Ilse, Raposo, Graca, Robine, Sylvie and Rivero, Francisco (2006) Brush border structure defects in I-plastin deficient mice European Journal of Cell Biology, 85, (Supplement 1), p.22. (doi:10.1016/j.ejcb.2006.02.011).

Record type: Article

Abstract

Plastin (or fi mbrin) is an actin-bundling protein consisting of two actin-binding domains in tandem preceded by two calcium-binding EF-hands. In mammals three different isoforms are expressed in a cell-type specifi c manner. Iplastin is specifi cally expressed in the small intestine, colon and kidney, where it localizes in microvilli, specialized surface structures with highly ordered microfi lament bundles. We have generated knockout mice lacking I-plastin. I-plastin defi cient mice showed no overt phenotype at the whole animal level: growth rate, reproductive rate and litter size were normal. Antibodies specifi c for T- and L-plastin were used to demonstrate that lacking of I-plastin is not compensated by another plastin isoform. Transmission electron microscopy studies revealed morphological differences between wildtype and I-plastin defi cient mice in the microvilli. Brush border preparations of knockout mice are sensitive and seem to degrade easily. In immunofl uorescence studies of intestinal mucosa cryosections decreased actin staining of the apical membrane was observed. Another abundant actin cross-linker of the brush border, villin, was found to be absent from the apical membrane. I-plastin seems to be an important regulator of the morphological structure and stability of microvilli.

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Published date: 1 March 2006

Identifiers

Local EPrints ID: 55963
URI: http://eprints.soton.ac.uk/id/eprint/55963
ISSN: 0171-9335
PURE UUID: e8cc8279-3845-4bd2-92e2-b1b68894e45d

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Date deposited: 17 Jul 2009
Last modified: 17 Jul 2017 14:31

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Contributors

Author: Eva-Maria S. Grimm-Günter
Author: Sonia Ramos
Author: Neil Smyth
Author: Revenu Celine
Author: Ilse Hurbain
Author: Graca Raposo
Author: Sylvie Robine
Author: Francisco Rivero

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