Brush border structure defects in I-plastin deficient mice

Grimm-Günter, Eva-Maria S., Ramos, Sonia, Smyth, Neil, Celine, Revenu, Hurbain, Ilse, Raposo, Graca, Robine, Sylvie and Rivero, Francisco (2006) Brush border structure defects in I-plastin deficient mice European Journal of Cell Biology, 85, (Supplement 1), p.22. (doi:10.1016/j.ejcb.2006.02.011).


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Plastin (or fi mbrin) is an actin-bundling protein consisting of two actin-binding domains in tandem preceded by two calcium-binding EF-hands. In mammals three different isoforms are expressed in a cell-type specifi c manner. Iplastin is specifi cally expressed in the small intestine, colon and kidney, where it localizes in microvilli, specialized surface structures with highly ordered microfi lament bundles. We have generated knockout mice lacking I-plastin. I-plastin defi cient mice showed no overt phenotype at the whole animal level: growth rate, reproductive rate and litter size were normal. Antibodies specifi c for T- and L-plastin were used to demonstrate that lacking of I-plastin is not compensated by another plastin isoform. Transmission electron microscopy studies revealed morphological differences between wildtype and I-plastin defi cient mice in the microvilli. Brush border preparations of knockout mice are sensitive and seem to degrade easily. In immunofl uorescence studies of intestinal mucosa cryosections decreased actin staining of the apical membrane was observed. Another abundant actin cross-linker of the brush border, villin, was found to be absent from the apical membrane. I-plastin seems to be an important regulator of the morphological structure and stability of microvilli.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/j.ejcb.2006.02.011
ISSNs: 0171-9335 (print)
Related URLs:
ePrint ID: 55963
Date :
Date Event
1 March 2006Published
Date Deposited: 17 Jul 2009
Last Modified: 16 Apr 2017 17:42
Further Information:Google Scholar

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