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Effects of artificial diet containing GNA and GNA-expressing potatoes on the development of the aphid parasitoid Aphidius ervi Haliday (Hymenoptera : Aphidiidae)

Effects of artificial diet containing GNA and GNA-expressing potatoes on the development of the aphid parasitoid Aphidius ervi Haliday (Hymenoptera : Aphidiidae)
Effects of artificial diet containing GNA and GNA-expressing potatoes on the development of the aphid parasitoid Aphidius ervi Haliday (Hymenoptera : Aphidiidae)
Aphid parasitoids are important biological control agents. The possibility arises that whilst foraging on insect-resistant transgenic plants, they are themselves at risk from direct and indirect effects of the expression of a transgene used to control the pest species. A liquid artificial diet was successfully used to deliver the snowdrop lectin (Galanthus nivalis agglutinin; GNA) to the peach-potato aphid, Myzus persicae. Bioassays utilising artificial diet incorporating GNA, and excised leaves of the GNA-expressing transgenic potato line, GNA2#28, were performed to assess the potential effects of GNA on the development of the aphid parasitoid Aphidius ervi. The results indicate that GNA delivered via artificial diet to the aphids can be transferred through the trophic levels and has a dose-dependent effect on parasitoid development. Parasitoid larvae excreted most of the ingested GNA in the meconium but some of it was detected in the pupae. Although A. ervi development was not affected when developing within hosts feeding on transgenic potato leaves, this probably reflected sub-optimal expression of the toxin in the transgenic potato line used
transgenic potato, galanthus nivalis agglutinin, aphid/parasitoid interaction, aphidiidae, integrated pest mangement, risk-assessment
0022-1910
1357-1366
Couty, A.
aaa8e364-e9b9-4388-8b81-2e6370d968a1
Down, R.E.
c8bc3201-c8dc-4707-b1d6-dd54d03189b9
Gatehouse, A.M.R.
0b634953-b260-4564-92ac-a00deb299698
Kaiser, L.
452fd293-1bdf-427c-bfc1-3f9ed500e912
Pham-Delegue, M.H.
70171b25-2797-4959-9484-18d692e0d64b
Poppy, G.M.
e18524cf-10ae-4ab4-b50c-e73e7d841389
Couty, A.
aaa8e364-e9b9-4388-8b81-2e6370d968a1
Down, R.E.
c8bc3201-c8dc-4707-b1d6-dd54d03189b9
Gatehouse, A.M.R.
0b634953-b260-4564-92ac-a00deb299698
Kaiser, L.
452fd293-1bdf-427c-bfc1-3f9ed500e912
Pham-Delegue, M.H.
70171b25-2797-4959-9484-18d692e0d64b
Poppy, G.M.
e18524cf-10ae-4ab4-b50c-e73e7d841389

Couty, A., Down, R.E., Gatehouse, A.M.R., Kaiser, L., Pham-Delegue, M.H. and Poppy, G.M. (2001) Effects of artificial diet containing GNA and GNA-expressing potatoes on the development of the aphid parasitoid Aphidius ervi Haliday (Hymenoptera : Aphidiidae). Journal of Insect Physiology, 47 (12), 1357-1366. (doi:10.1016/S0022-1910(01)00111-1).

Record type: Article

Abstract

Aphid parasitoids are important biological control agents. The possibility arises that whilst foraging on insect-resistant transgenic plants, they are themselves at risk from direct and indirect effects of the expression of a transgene used to control the pest species. A liquid artificial diet was successfully used to deliver the snowdrop lectin (Galanthus nivalis agglutinin; GNA) to the peach-potato aphid, Myzus persicae. Bioassays utilising artificial diet incorporating GNA, and excised leaves of the GNA-expressing transgenic potato line, GNA2#28, were performed to assess the potential effects of GNA on the development of the aphid parasitoid Aphidius ervi. The results indicate that GNA delivered via artificial diet to the aphids can be transferred through the trophic levels and has a dose-dependent effect on parasitoid development. Parasitoid larvae excreted most of the ingested GNA in the meconium but some of it was detected in the pupae. Although A. ervi development was not affected when developing within hosts feeding on transgenic potato leaves, this probably reflected sub-optimal expression of the toxin in the transgenic potato line used

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More information

Submitted date: 27 November 2000
Published date: 1 December 2001
Keywords: transgenic potato, galanthus nivalis agglutinin, aphid/parasitoid interaction, aphidiidae, integrated pest mangement, risk-assessment

Identifiers

Local EPrints ID: 55982
URI: https://eprints.soton.ac.uk/id/eprint/55982
ISSN: 0022-1910
PURE UUID: 4b5b3000-af5b-4574-a744-58dc657d4fad

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Date deposited: 08 Aug 2008
Last modified: 13 Mar 2019 20:35

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Contributors

Author: A. Couty
Author: R.E. Down
Author: A.M.R. Gatehouse
Author: L. Kaiser
Author: M.H. Pham-Delegue
Author: G.M. Poppy

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