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Stable recognition of TA interruptions by triplex forming oligonucleotides containing a novel nucleoside

Stable recognition of TA interruptions by triplex forming oligonucleotides containing a novel nucleoside
Stable recognition of TA interruptions by triplex forming oligonucleotides containing a novel nucleoside
We have prepared the 2'-aminoethoxy derivative of the S nucleoside (2AES) and incorporated it into triplex-forming oligonucleotides for recognition of TA interruptions within a target oligopurine tract. Fluorescence melting, UV melting, and DNase I footprinting experiments show that 2AES has greater affinity than G or S for a single TA interruption. Stable triplexes are formed at pH 6.0 at an 18-mer target site containing two TA interruptions, even though this contains eight C+.GC triplets. Although 2AES and S produce stable triplexes at TA interruptions, they also interact with other base pairs, in particular, CG, although the selectivity for TA improves with increased pH. 2AES is the best nucleoside described so far for recognition of TA within a triple-helix target.
0006-2960
5884-5892
Wang, Yang
7bfb9a35-82f9-4580-a448-c4fbffc7959c
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Powers, Vicki E.C.
0646d625-5039-454c-a8ad-0da6bc72ae8a
Lack, Oliver
cfbb55aa-1dbb-4088-bb67-0d58f7aa07a0
Osborne, Sadie D.
9777c83c-8998-4fd6-913e-04d3902ee9cd
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Wang, Yang
7bfb9a35-82f9-4580-a448-c4fbffc7959c
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Powers, Vicki E.C.
0646d625-5039-454c-a8ad-0da6bc72ae8a
Lack, Oliver
cfbb55aa-1dbb-4088-bb67-0d58f7aa07a0
Osborne, Sadie D.
9777c83c-8998-4fd6-913e-04d3902ee9cd
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Wang, Yang, Rusling, David A., Powers, Vicki E.C., Lack, Oliver, Osborne, Sadie D., Fox, Keith R. and Brown, Tom (2005) Stable recognition of TA interruptions by triplex forming oligonucleotides containing a novel nucleoside. Biochemistry, 44 (15), 5884-5892. (doi:10.1021/bi050013v).

Record type: Article

Abstract

We have prepared the 2'-aminoethoxy derivative of the S nucleoside (2AES) and incorporated it into triplex-forming oligonucleotides for recognition of TA interruptions within a target oligopurine tract. Fluorescence melting, UV melting, and DNase I footprinting experiments show that 2AES has greater affinity than G or S for a single TA interruption. Stable triplexes are formed at pH 6.0 at an 18-mer target site containing two TA interruptions, even though this contains eight C+.GC triplets. Although 2AES and S produce stable triplexes at TA interruptions, they also interact with other base pairs, in particular, CG, although the selectivity for TA improves with increased pH. 2AES is the best nucleoside described so far for recognition of TA within a triple-helix target.

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More information

Submitted date: 4 January 2005
Published date: 1 April 2005

Identifiers

Local EPrints ID: 55986
URI: https://eprints.soton.ac.uk/id/eprint/55986
ISSN: 0006-2960
PURE UUID: 0cd585f2-17fc-4cfd-90ff-95dd079f3fcd
ORCID for David A. Rusling: ORCID iD orcid.org/0000-0002-7442-686X
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 06 Jun 2018 13:16

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