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Fluorescence in situ hybridization using peptide nucleic acid probes for rapid detection of Mycobacterium avium subsp avium and Mycobacterium avium subsp paratuberculosis in potable-water biofilms

Lehtola, M.J., Torvinen, E., Miettinen, L.T. and Keevil, C.W. (2006) Fluorescence in situ hybridization using peptide nucleic acid probes for rapid detection of Mycobacterium avium subsp avium and Mycobacterium avium subsp paratuberculosis in potable-water biofilms Applied and Environmental Microbiology, 72, (1), pp. 848-853. (doi:10.1128/AEM.72.1.848-853.2006).

Record type: Article

Abstract

Here, we present for the first time a high-affinity peptide nucleic acid (PNA) oligonucleotide sequence for detecting Mycobacterium avium bacteria, including the opportunistically pathogenic subspecies M. avium subsp. avium, M. avium subsp. paratuberculosis, and M. avium subsp. silvaticum, by the fluorescence in situ hybridization (FISH) method. There is evidence that M. avium subsp. avium especially is able to survive and grow in drinking-water biofilms and possibly transmit via drinking water. The designed PNA probe (MAV148) specificity was tested with several bacterial species, including other mycobacteria and mycolic acid-containing bacteria. From the range of bacterial strains tested, only M. avium subsp. avium and M. avium subsp. paratuberculosis strains were hybridized. The PNA FISH method was applied successfully to detect M. avium subsp. avium spiked in water samples and biofilm established within a Propella biofilm reactor fed with potable water from a distribution supply.

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More information

Submitted date: 30 May 2005
Published date: 1 January 2006

Identifiers

Local EPrints ID: 55990
URI: http://eprints.soton.ac.uk/id/eprint/55990
ISSN: 0099-2240
PURE UUID: 591ad20b-630c-40f1-a027-f7a289d4d1d6
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 17 Jul 2017 14:31

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Contributors

Author: M.J. Lehtola
Author: E. Torvinen
Author: L.T. Miettinen
Author: C.W. Keevil ORCID iD

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