The University of Southampton
University of Southampton Institutional Repository

Development and application of a novel peptide nucleic acid (PNA) probe for the specific detection of Helicobacter pylori

Record type: Article

The standard methods for accurate diagnosis of Helicobacter pylori infection consist in either culturing of the pathogen and/or concordant positive results obtained by histology and the rapid urease test or the 13C-urea breath test (UBT), as none of these diagnostic tests except bacterial culturing are 100% specific, fluorescence in situ hybridization (FISH) might be a good complement. In this work, the development of a new peptide nucleic acid (PNA) FISH probe for the detection of H. pylori is reported. The 15-mer PNA probe was connected to an Alexa Fluor 546 dye and target a specific 16S rRNA sequence of the bacterium. PNA probes are a recent technology that has been shown to provide for additional specificity and sensitivity in FISH procedures when compared to the standard DNA counterparts. The probe was tested against several H. pylori and non-H. pylori strains, and was shown to be specific for the microorganism of interest. This technique was optimized for different types of supports such as slides, membrane filters, and also coupons of various materials where H. pylori was present. Tests performed showed a better sensitivity of the probe than the standard plating procedures for H. pylori detection. We are currently optimizing the application of this new probe for paraffin-embedded gastric biopsy specimens. When completely optimized, this technique could be a useful method, as it is rapid, sensitive, and specific, for an even more accurate diagnostic of H. pylori infection, and could also be used for distinguishing H. pylori from other Helicobacter species.

Full text not available from this repository.

Citation

Guimaraes, N.M., Azevedo, N.F., Figueiredo, C., Keevil, C.W. and Vieira, M.J. (2006) Development and application of a novel peptide nucleic acid (PNA) probe for the specific detection of Helicobacter pylori Helicobacter, 11, (Abs.10.10), p. 383. (doi:10.1111/j.1523-5378.2006.00419_12.x).

More information

Published date: 1 August 2006

Identifiers

Local EPrints ID: 56010
URI: http://eprints.soton.ac.uk/id/eprint/56010
ISSN: 1083-4389
PURE UUID: cc8f12b1-47de-4110-a524-16047ba8add9
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:31

Export record

Altmetrics

Contributors

Author: N.M. Guimaraes
Author: N.F. Azevedo
Author: C. Figueiredo
Author: C.W. Keevil ORCID iD
Author: M.J. Vieira

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×