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Structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase complexed with a diacid inhibitor

Structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase complexed with a diacid inhibitor
Structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase complexed with a diacid inhibitor
The structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase (ALAD) complexed with the irreversible inhibitor 4,7-dioxosebacic acid has been solved. The inhibitor binds by forming Schiff-base linkages with lysines 200 and 253 at the active site. The structure reported here provides a definition of the interactions made by both of the substrate molecules (A-side and P-side substrates) with the C. vibrioforme ALAD and is compared and contrasted with structures of the same inhibitor bound to Escherichia coli and yeast ALAD. The structure suggests why 4,7-dioxosebacic acid is a better inhibitor of the zinc-dependent ALADs than of the zinc-independent ALADs.
5-aminolaevulinate dehydratase, 4, 7-dioxosebacic acid.
0907-4449
1594-1598
Coates, L.
2be36c11-8cb2-4518-a001-953051aa6a23
Beaven, G.
d9cfa85e-3ea5-4ebe-8d9b-4a2db1ceb387
Erskine, P.T.
c77b60c5-b80c-4e6a-a103-bf57ecfcbcf6
Beale, S.I.
e056f8b5-e539-4935-b95e-210b18888eb7
Wood, S.P.
430faabf-7f5c-4cf6-9bcc-5955f5e09566
Shoolingin-Jordan, P.M.
ac0bf2cc-ee36-4b30-bcef-525cee2559f7
Cooper, J.B.
d9f0f6a8-1260-48fc-aa5c-3dbc650e3ec0
Coates, L.
2be36c11-8cb2-4518-a001-953051aa6a23
Beaven, G.
d9cfa85e-3ea5-4ebe-8d9b-4a2db1ceb387
Erskine, P.T.
c77b60c5-b80c-4e6a-a103-bf57ecfcbcf6
Beale, S.I.
e056f8b5-e539-4935-b95e-210b18888eb7
Wood, S.P.
430faabf-7f5c-4cf6-9bcc-5955f5e09566
Shoolingin-Jordan, P.M.
ac0bf2cc-ee36-4b30-bcef-525cee2559f7
Cooper, J.B.
d9f0f6a8-1260-48fc-aa5c-3dbc650e3ec0

Coates, L., Beaven, G., Erskine, P.T., Beale, S.I., Wood, S.P., Shoolingin-Jordan, P.M. and Cooper, J.B. (2005) Structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase complexed with a diacid inhibitor. Acta Crystallographica Section D: Biological Crystallography, 61, 1594-1598. (doi:10.1107/S0907444905030350).

Record type: Article

Abstract

The structure of Chlorobium vibrioforme 5-aminolaevulinic acid dehydratase (ALAD) complexed with the irreversible inhibitor 4,7-dioxosebacic acid has been solved. The inhibitor binds by forming Schiff-base linkages with lysines 200 and 253 at the active site. The structure reported here provides a definition of the interactions made by both of the substrate molecules (A-side and P-side substrates) with the C. vibrioforme ALAD and is compared and contrasted with structures of the same inhibitor bound to Escherichia coli and yeast ALAD. The structure suggests why 4,7-dioxosebacic acid is a better inhibitor of the zinc-dependent ALADs than of the zinc-independent ALADs.

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More information

Published date: 1 December 2005
Keywords: 5-aminolaevulinate dehydratase, 4, 7-dioxosebacic acid.

Identifiers

Local EPrints ID: 56032
URI: https://eprints.soton.ac.uk/id/eprint/56032
ISSN: 0907-4449
PURE UUID: 184181c0-ab63-4331-ac8e-a6fd5df488ab

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 13 Mar 2019 20:35

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