The University of Southampton
University of Southampton Institutional Repository

The electrophoretic mobility of DNA three-way junctions is affected by the sequence of overhanging single-stranded ends

The electrophoretic mobility of DNA three-way junctions is affected by the sequence of overhanging single-stranded ends
The electrophoretic mobility of DNA three-way junctions is affected by the sequence of overhanging single-stranded ends
The folding of three- and four-way DNA junctions is often assessed by comparing the electrophoretic mobility of restriction enzyme fragments, using the long-short arm assay. We have compared the mobility of synthetic three-way junctions that contain identical branch point sequences, but different restriction sites in the arms. We show that the mobility of fragments is affected by the sequence of the overhanging ends. In general, GC-rich overhangs produce fragments with anomalous mobilities. These anomalies can be prevented by treating the cleaved junctions with mung bean endonuclease, elevating the electrophoresis temperature or using blunt cleaving restriction endonucleases
gel mobility, three-way DNA junctions
0173-0835
413-417
Assenberg, R.
665b7d5b-4bc7-47f6-ba77-17e15d9103d3
Fox, K.R.
9da5debc-4e45-473e-ab8c-550d1104659f
Assenberg, R.
665b7d5b-4bc7-47f6-ba77-17e15d9103d3
Fox, K.R.
9da5debc-4e45-473e-ab8c-550d1104659f

Assenberg, R. and Fox, K.R. (2001) The electrophoretic mobility of DNA three-way junctions is affected by the sequence of overhanging single-stranded ends. Electrophoresis, 22 (3), 413-417. (doi:10.1002/1522-2683(200102)22:3<413::AID-ELPS413>3.0.CO;2-S).

Record type: Article

Abstract

The folding of three- and four-way DNA junctions is often assessed by comparing the electrophoretic mobility of restriction enzyme fragments, using the long-short arm assay. We have compared the mobility of synthetic three-way junctions that contain identical branch point sequences, but different restriction sites in the arms. We show that the mobility of fragments is affected by the sequence of the overhanging ends. In general, GC-rich overhangs produce fragments with anomalous mobilities. These anomalies can be prevented by treating the cleaved junctions with mung bean endonuclease, elevating the electrophoresis temperature or using blunt cleaving restriction endonucleases

This record has no associated files available for download.

More information

Published date: 1 February 2001
Keywords: gel mobility, three-way DNA junctions

Identifiers

Local EPrints ID: 56037
URI: http://eprints.soton.ac.uk/id/eprint/56037
ISSN: 0173-0835
PURE UUID: 03153507-ca55-4823-8c6a-79ff5fcf5b3e
ORCID for K.R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 08 Aug 2008
Last modified: 16 Mar 2024 02:36

Export record

Altmetrics

Contributors

Author: R. Assenberg
Author: K.R. Fox ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×