Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms
Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms
Close association of the pathogen Helicobacter pylori in drinking water biofilms has been suggested. Using a two-stage water model, the survival and development of the pathogen in potable water biofilms was monitored. Filter sterilized tap water was used as the growth medium and the inoculum consisted of a naturally occurring consortium of microorganisms. Biofilms were generated on removable stainless steel coupons that were placed in the second vessel. Novel technology peptide nucleic acid (PNA) molecular probes were used to detect and locate the pathogen in the biofilms. The PNA-labelled oligonucleotide probes were highly specific, and complementary to the helix 6 region of H. pylori 16S rRNA. The pathogen was tracked in the biofilms using epifluorescence microscopy and episcopic differential interference contrast microscopy. Results show that H. pylori can successfully incorporate within biofilms and its presence was detected for up to five days after inoculation. PNA probes provided an easy and quick way of performing fluorescence in situ hybridisation assays in heterogeneous biofilms.
155-160
Azevedo, N.F.
c90d7c41-e45a-404d-9472-9d0b411448e7
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
1 January 2003
Azevedo, N.F.
c90d7c41-e45a-404d-9472-9d0b411448e7
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Azevedo, N.F., Vieira, M.J. and Keevil, C.W.
(2003)
Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms.
Water Science & Technology, 47 (5), .
Abstract
Close association of the pathogen Helicobacter pylori in drinking water biofilms has been suggested. Using a two-stage water model, the survival and development of the pathogen in potable water biofilms was monitored. Filter sterilized tap water was used as the growth medium and the inoculum consisted of a naturally occurring consortium of microorganisms. Biofilms were generated on removable stainless steel coupons that were placed in the second vessel. Novel technology peptide nucleic acid (PNA) molecular probes were used to detect and locate the pathogen in the biofilms. The PNA-labelled oligonucleotide probes were highly specific, and complementary to the helix 6 region of H. pylori 16S rRNA. The pathogen was tracked in the biofilms using epifluorescence microscopy and episcopic differential interference contrast microscopy. Results show that H. pylori can successfully incorporate within biofilms and its presence was detected for up to five days after inoculation. PNA probes provided an easy and quick way of performing fluorescence in situ hybridisation assays in heterogeneous biofilms.
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Published date: 1 January 2003
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Local EPrints ID: 56067
URI: http://eprints.soton.ac.uk/id/eprint/56067
ISSN: 0273-1223
PURE UUID: 40223073-0a9b-4211-8a86-d50644e76d4c
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Date deposited: 07 Aug 2008
Last modified: 09 Jan 2022 03:08
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Author:
N.F. Azevedo
Author:
M.J. Vieira
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