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Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms

Azevedo, N.F., Vieira, M.J. and Keevil, C.W. (2003) Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms Water Science & Technology, 47, (5), pp. 155-160.

Record type: Article


Close association of the pathogen Helicobacter pylori in drinking water biofilms has been suggested. Using a two-stage water model, the survival and development of the pathogen in potable water biofilms was monitored. Filter sterilized tap water was used as the growth medium and the inoculum consisted of a naturally occurring consortium of microorganisms. Biofilms were generated on removable stainless steel coupons that were placed in the second vessel. Novel technology peptide nucleic acid (PNA) molecular probes were used to detect and locate the pathogen in the biofilms. The PNA-labelled oligonucleotide probes were highly specific, and complementary to the helix 6 region of H. pylori 16S rRNA. The pathogen was tracked in the biofilms using epifluorescence microscopy and episcopic differential interference contrast microscopy. Results show that H. pylori can successfully incorporate within biofilms and its presence was detected for up to five days after inoculation. PNA probes provided an easy and quick way of performing fluorescence in situ hybridisation assays in heterogeneous biofilms.

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Published date: 1 January 2003


Local EPrints ID: 56067
ISSN: 0273-1223
PURE UUID: 40223073-0a9b-4211-8a86-d50644e76d4c
ORCID for C.W. Keevil: ORCID iD

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Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:31

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Author: N.F. Azevedo
Author: M.J. Vieira
Author: C.W. Keevil ORCID iD

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