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Role of a FMRFamide-like family of neuropeptides in the pharyngeal nervous system of Caenorhabditis elegans

Role of a FMRFamide-like family of neuropeptides in the pharyngeal nervous system of Caenorhabditis elegans
Role of a FMRFamide-like family of neuropeptides in the pharyngeal nervous system of Caenorhabditis elegans
The nervous system of C. elegans has a remarkable abundance of flp genes encoding FMRFamide-like (FLP) neuropeptides. To provide insight into the physiological relevance of this neuropeptide diversity, we have tested more than 30 FLPs (encoded by 23 flps) for bioactivity on C. elegans pharynx. Eleven flp genes encode peptides that inhibit pharyngeal activity, while eight flp genes encode peptides that are excitatory. Three potent peptides (inhibitory, FLP-13A, APEASPFIRFamide; excitatory, FLP-17A, KSAFVRFamide; excitatory, FLP-17B, KSQYIRFamide) are encoded by flp genes, which, according to reporter gene constructs, are expressed in pharyngeal motoneurons. Thus, they may act through receptors localized on the pharyngeal muscle. The two other potent peptides, FLP-8 (excitatory AF1, KNEFIRFamide,) and FLP-11A (inhibitory, AMRNALVRFamide), appear to be expressed in extrapharyngeal neurons and are therefore likely to act either indirectly or as neurohormones. Intriguingly, a single neuron can express peptides that have potent but opposing biological activity in the pharynx. Only five flp genes encode neuropeptides that have no observable effect on the pharynx, but none of these have shown reporter gene expression in the pharyngeal nervous system. To examine the roles of multiple peptides produced from single precursors, a comparison was made between the bioactivity of different neuropeptides for five flp genes (flp-3, flp-13, flp-14, flp-17, and flp-18). For all but one gene (flp-14), the effects of peptides encoded by the same gene were similar. Overall, this study demonstrates the impressive neurochemical complexity of the simple circuit that regulates feeding in the nematode, C. elegans. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2005
nematode, Caenorhabditis elegans, pharynx, neuropeptides, RFamide
0022-3034
304-319
Papaioannou, Sylvana
f125366e-9363-49ac-ab2d-87377e82d09a
Marsden, David
18ea64e4-9ba5-4f57-b19d-f874589279b6
Franks, Christopher J.
9842534b-4d3f-4ee8-a07e-3b050f748593
Walker, Robert J.
9368ac2d-f1e9-4bd9-a4b4-4a161c4aa140
Holden-Dye, Lindy
8032bf60-5db6-40cb-b71c-ddda9d212c8e
Papaioannou, Sylvana
f125366e-9363-49ac-ab2d-87377e82d09a
Marsden, David
18ea64e4-9ba5-4f57-b19d-f874589279b6
Franks, Christopher J.
9842534b-4d3f-4ee8-a07e-3b050f748593
Walker, Robert J.
9368ac2d-f1e9-4bd9-a4b4-4a161c4aa140
Holden-Dye, Lindy
8032bf60-5db6-40cb-b71c-ddda9d212c8e

Papaioannou, Sylvana, Marsden, David, Franks, Christopher J., Walker, Robert J. and Holden-Dye, Lindy (2005) Role of a FMRFamide-like family of neuropeptides in the pharyngeal nervous system of Caenorhabditis elegans. Journal of Neurobiology, 65 (3), 304-319. (doi:10.1002/neu.20201).

Record type: Article

Abstract

The nervous system of C. elegans has a remarkable abundance of flp genes encoding FMRFamide-like (FLP) neuropeptides. To provide insight into the physiological relevance of this neuropeptide diversity, we have tested more than 30 FLPs (encoded by 23 flps) for bioactivity on C. elegans pharynx. Eleven flp genes encode peptides that inhibit pharyngeal activity, while eight flp genes encode peptides that are excitatory. Three potent peptides (inhibitory, FLP-13A, APEASPFIRFamide; excitatory, FLP-17A, KSAFVRFamide; excitatory, FLP-17B, KSQYIRFamide) are encoded by flp genes, which, according to reporter gene constructs, are expressed in pharyngeal motoneurons. Thus, they may act through receptors localized on the pharyngeal muscle. The two other potent peptides, FLP-8 (excitatory AF1, KNEFIRFamide,) and FLP-11A (inhibitory, AMRNALVRFamide), appear to be expressed in extrapharyngeal neurons and are therefore likely to act either indirectly or as neurohormones. Intriguingly, a single neuron can express peptides that have potent but opposing biological activity in the pharynx. Only five flp genes encode neuropeptides that have no observable effect on the pharynx, but none of these have shown reporter gene expression in the pharyngeal nervous system. To examine the roles of multiple peptides produced from single precursors, a comparison was made between the bioactivity of different neuropeptides for five flp genes (flp-3, flp-13, flp-14, flp-17, and flp-18). For all but one gene (flp-14), the effects of peptides encoded by the same gene were similar. Overall, this study demonstrates the impressive neurochemical complexity of the simple circuit that regulates feeding in the nematode, C. elegans. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2005

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More information

Published date: December 2005
Keywords: nematode, Caenorhabditis elegans, pharynx, neuropeptides, RFamide

Identifiers

Local EPrints ID: 56127
URI: https://eprints.soton.ac.uk/id/eprint/56127
ISSN: 0022-3034
PURE UUID: 427e2813-95c5-49c6-ade5-a433e345d838
ORCID for Christopher J. Franks: ORCID iD orcid.org/0000-0002-5412-7037
ORCID for Robert J. Walker: ORCID iD orcid.org/0000-0002-9031-7671

Catalogue record

Date deposited: 07 Aug 2008
Last modified: 14 Mar 2019 01:51

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