The University of Southampton
University of Southampton Institutional Repository

Structure determination of uniformly labelled ligands in receptor systems: Acetylcholine - A case study

Record type: Article

Recent developments in SS-MAS-NMR methodology have brought about a wealth of information regarding the conformation and environment of small molecules bound to membrane bound receptors. Currently the application of these methods to structure determination in biological systems has relied extensively on the ability to introduce isolated spin pairs and the subsequent determination of single internuclear distances using techniques such as rotational resonance or REDOR. We present preliminary data on the application of rotational resonance experiments to the study of uniformly labelled molecules. Qualitatively the data obtained is similar to that expected from systems labelled at unique sites. Analysis of such exchange curves obtained for a uniformly labelled acetylcholine perchlorate, using a model derived for isolated spin pairs, shows good quantitative agreement between the observed geometries and those found in the crystal structure. Such experiments may offer an alternative to selective labelling in some systems, reducing the time and expense associated with the preparation of multiple samples containing isolated spin pairs necessary for these studies Progress is currently being made in the application of these techniques to the study of acetylcholine whilst bound to the nAcChR, with the aim of defining the global conformation of the molecule whilst bound to the receptor

Full text not available from this repository.

Citation

Williamson, P.T.F., Verhoeven, A., Miller, K., Watts, A. and Meier, B. (2002) Structure determination of uniformly labelled ligands in receptor systems: Acetylcholine - A case study Biophysical Journal, 82, (1), 518A-519A.

More information

Published date: 1 January 2002

Identifiers

Local EPrints ID: 56138
URI: http://eprints.soton.ac.uk/id/eprint/56138
ISSN: 0006-3495
PURE UUID: b3f35782-b316-4913-b728-4f582112501a
ORCID for P.T.F. Williamson: ORCID iD orcid.org/0000-0002-0231-8640

Catalogue record

Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:31

Export record

Contributors

Author: A. Verhoeven
Author: K. Miller
Author: A. Watts
Author: B. Meier

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×