12-O-Tetradecanoyl-phorbol-13-acetate down-regulates the Huntingtin promoter at Spl sites
12-O-Tetradecanoyl-phorbol-13-acetate down-regulates the Huntingtin promoter at Spl sites
We have studied the effects of the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on Huntington's disease (HD) gene transcription in neuronal and non-neuronal cell lines, to investigate pathways regulating HD gene expression. TPA reduced transcription from the HD gene promoter in SKN-SH (neuroblastoma) and HeLa cells but not in JEG3 (choriocarcinoma) cells. In SK-N-SH cells, the responsible cis-acting promoter sequences comprise the tandemly duplicated Spl sites in the region from -213 to -174, relative to the translation start site. The TPA-down-regulating region in HeLa cells was mapped to the sequence from -141 to -126. In conclusion, this demonstrates that HD gene transcription can be down-regulated in vitro in a cell-specific manner.
3157-3161
Coles, Rhian
3e67cc1a-77e5-456e-905c-09b750e86905
Birdsall, Michelle
1b12bdb6-6ab4-42a4-a98e-f0b661850398
Wyttenbach, Andreas
05019897-52b1-4bb6-b259-5d51abae7540
Rubinsztein, David C.
c841997b-4a3f-4643-b215-0f206d29a39e
28 September 2000
Coles, Rhian
3e67cc1a-77e5-456e-905c-09b750e86905
Birdsall, Michelle
1b12bdb6-6ab4-42a4-a98e-f0b661850398
Wyttenbach, Andreas
05019897-52b1-4bb6-b259-5d51abae7540
Rubinsztein, David C.
c841997b-4a3f-4643-b215-0f206d29a39e
Coles, Rhian, Birdsall, Michelle, Wyttenbach, Andreas and Rubinsztein, David C.
(2000)
12-O-Tetradecanoyl-phorbol-13-acetate down-regulates the Huntingtin promoter at Spl sites.
NeuroReport, 11 (14), .
Abstract
We have studied the effects of the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on Huntington's disease (HD) gene transcription in neuronal and non-neuronal cell lines, to investigate pathways regulating HD gene expression. TPA reduced transcription from the HD gene promoter in SKN-SH (neuroblastoma) and HeLa cells but not in JEG3 (choriocarcinoma) cells. In SK-N-SH cells, the responsible cis-acting promoter sequences comprise the tandemly duplicated Spl sites in the region from -213 to -174, relative to the translation start site. The TPA-down-regulating region in HeLa cells was mapped to the sequence from -141 to -126. In conclusion, this demonstrates that HD gene transcription can be down-regulated in vitro in a cell-specific manner.
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Published date: 28 September 2000
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Local EPrints ID: 56151
URI: http://eprints.soton.ac.uk/id/eprint/56151
PURE UUID: 64f99aa9-7c34-488a-b594-8f089d5cc462
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Date deposited: 21 Aug 2008
Last modified: 11 Dec 2021 17:47
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Author:
Rhian Coles
Author:
Michelle Birdsall
Author:
Andreas Wyttenbach
Author:
David C. Rubinsztein
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