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Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH

Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH
Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH
We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurine · oligopyrimidine sequence, using oligonucleotides that contain combinations of 2?-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) and 3-methyl-2-aminopyridine (MeP) in place of T and C, respectively. This combination acts cooperatively to enable high affinity triple helix formation at physiological pH. The affinity depends on the number of substitutions and their arrangement; oligonucleotides in which these analogues are evenly distributed throughout the third strand bind much better than those in which they are clustered together.
triple helix, nucleoside analogue, footprinting, dna sequence recognition
0014-5793
6616-6620
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Le Strat, Loic
46c7e010-1cf4-4022-8017-a126844d4a04
Powers, Vicki E.C.
0646d625-5039-454c-a8ad-0da6bc72ae8a
Broughton-Head, Victoria J.
d094aa77-c889-4747-84d2-55542805c67c
Booth, James
ce6cfba6-ac11-4b72-afc4-0f15930d0a1a
Lack, Oliver
cfbb55aa-1dbb-4088-bb67-0d58f7aa07a0
Brown, Tom
1cd7df32-b945-4ca1-8b59-a51a30191472
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Rusling, David A.
d08f1f97-f8a9-4980-a025-ae41c23a938f
Le Strat, Loic
46c7e010-1cf4-4022-8017-a126844d4a04
Powers, Vicki E.C.
0646d625-5039-454c-a8ad-0da6bc72ae8a
Broughton-Head, Victoria J.
d094aa77-c889-4747-84d2-55542805c67c
Booth, James
ce6cfba6-ac11-4b72-afc4-0f15930d0a1a
Lack, Oliver
cfbb55aa-1dbb-4088-bb67-0d58f7aa07a0
Brown, Tom
1cd7df32-b945-4ca1-8b59-a51a30191472
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f

Rusling, David A., Le Strat, Loic, Powers, Vicki E.C., Broughton-Head, Victoria J., Booth, James, Lack, Oliver, Brown, Tom and Fox, Keith R. (2005) Combining nucleoside analogues to achieve recognition of oligopurine tracts by triplex-forming oligonucleotides at physiological pH. FEBS Letters, 579 (29), 6616-6620. (doi:10.1016/j.febslet.2005.10.056).

Record type: Article

Abstract

We have used DNase I footprinting to examine DNA triple helix formation at a 12 base pair oligopurine · oligopyrimidine sequence, using oligonucleotides that contain combinations of 2?-aminoethoxy-5-(3-aminoprop-1-ynyl)uridine (bis-amino-U, BAU) and 3-methyl-2-aminopyridine (MeP) in place of T and C, respectively. This combination acts cooperatively to enable high affinity triple helix formation at physiological pH. The affinity depends on the number of substitutions and their arrangement; oligonucleotides in which these analogues are evenly distributed throughout the third strand bind much better than those in which they are clustered together.

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More information

Submitted date: 11 August 2005
Published date: 5 December 2005
Keywords: triple helix, nucleoside analogue, footprinting, dna sequence recognition

Identifiers

Local EPrints ID: 56158
URI: https://eprints.soton.ac.uk/id/eprint/56158
ISSN: 0014-5793
PURE UUID: d80877b8-8a06-453a-bd3a-b3e31bb96485
ORCID for David A. Rusling: ORCID iD orcid.org/0000-0002-7442-686X
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 07 Aug 2008
Last modified: 14 Mar 2019 01:56

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Contributors

Author: Loic Le Strat
Author: Vicki E.C. Powers
Author: Victoria J. Broughton-Head
Author: James Booth
Author: Oliver Lack
Author: Tom Brown
Author: Keith R. Fox ORCID iD

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