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Differential regulation of type I and type II interleukin-1 receptors in focal brain inflammation

Differential regulation of type I and type II interleukin-1 receptors in focal brain inflammation
Differential regulation of type I and type II interleukin-1 receptors in focal brain inflammation
Most pathologies of the brain have an inflammatory component, associated with the release of cytokines such as interleukin-1? (IL-1?) from resident and infiltrating cells. The IL-1 type I receptor (IL-1RI) initiates a signalling cascade but the type II receptor (IL-1RII) acts as a decoy receptor. Here we have investigated the expression of IL-1?, IL-1RI and IL-1RII in distinct inflammatory lesions in the rat brain. IL-1? was injected into the brain to generate an inflammatory lesion in the absence of neuronal cell death whereas neuronal death was specifically induced by the microinjection of N-methyl-d-aspartate (NMDA). Using TaqMan RT-PCR and ELISA, we observed elevated de novo IL-1? synthesis 2 h after the intracerebral microinjection of IL-1?; this de novo IL-1? remained elevated 24 h later. There was a concomitant increase in IL-1RI mRNA but a much greater increase in IL-1RII mRNA. Immunostaining revealed that IL-1RII was expressed on brain endothelial cells and on infiltrating neutrophils. In contrast, although IL-1? and IL-1RI were elevated to similar levels in response to NMDA challenge, the response was delayed and IL-1RII mRNA expression was unchanged. The lesion-specific expression of IL-1 receptors suggests that the receptors are differentially regulated in a manner not directly related to the endogenous level of IL-1 in the CNS.
excitotoxicity, neuroinflammation, Rattus norvegicus, TaqMan RT-PCR
0953-816X
1205-1214
Docagne, F.
5997c6a4-db39-4216-b41b-e0bcf29b6cf2
Campbell, S.J.
fe349c31-dac5-42fb-ae2c-0f841b145083
Bristow, A.F.
6b797abf-29b0-4abb-9e98-34e9ada2fc3f
Poole, S.
4e7e9a5a-df95-4f65-a21d-00d7dfcd5019
Vigues, S.
efb61aa5-1e9d-4046-bb8b-8bfdb6e5bf27
Guaza, C.
30974ab9-2da9-439e-b625-f59ebda6c937
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
Anthony, D.C.
70fb8e27-4e74-4c72-b1b5-3a4ca0d6b8cf
Docagne, F.
5997c6a4-db39-4216-b41b-e0bcf29b6cf2
Campbell, S.J.
fe349c31-dac5-42fb-ae2c-0f841b145083
Bristow, A.F.
6b797abf-29b0-4abb-9e98-34e9ada2fc3f
Poole, S.
4e7e9a5a-df95-4f65-a21d-00d7dfcd5019
Vigues, S.
efb61aa5-1e9d-4046-bb8b-8bfdb6e5bf27
Guaza, C.
30974ab9-2da9-439e-b625-f59ebda6c937
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
Anthony, D.C.
70fb8e27-4e74-4c72-b1b5-3a4ca0d6b8cf

Docagne, F., Campbell, S.J., Bristow, A.F., Poole, S., Vigues, S., Guaza, C., Perry, V.H. and Anthony, D.C. (2005) Differential regulation of type I and type II interleukin-1 receptors in focal brain inflammation. European Journal of Neuroscience, 21 (5), 1205-1214. (doi:10.1111/j.1460-9568.2005.03965.x).

Record type: Article

Abstract

Most pathologies of the brain have an inflammatory component, associated with the release of cytokines such as interleukin-1? (IL-1?) from resident and infiltrating cells. The IL-1 type I receptor (IL-1RI) initiates a signalling cascade but the type II receptor (IL-1RII) acts as a decoy receptor. Here we have investigated the expression of IL-1?, IL-1RI and IL-1RII in distinct inflammatory lesions in the rat brain. IL-1? was injected into the brain to generate an inflammatory lesion in the absence of neuronal cell death whereas neuronal death was specifically induced by the microinjection of N-methyl-d-aspartate (NMDA). Using TaqMan RT-PCR and ELISA, we observed elevated de novo IL-1? synthesis 2 h after the intracerebral microinjection of IL-1?; this de novo IL-1? remained elevated 24 h later. There was a concomitant increase in IL-1RI mRNA but a much greater increase in IL-1RII mRNA. Immunostaining revealed that IL-1RII was expressed on brain endothelial cells and on infiltrating neutrophils. In contrast, although IL-1? and IL-1RI were elevated to similar levels in response to NMDA challenge, the response was delayed and IL-1RII mRNA expression was unchanged. The lesion-specific expression of IL-1 receptors suggests that the receptors are differentially regulated in a manner not directly related to the endogenous level of IL-1 in the CNS.

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More information

Submitted date: 5 October 2004
Published date: 1 March 2005
Keywords: excitotoxicity, neuroinflammation, Rattus norvegicus, TaqMan RT-PCR

Identifiers

Local EPrints ID: 56177
URI: https://eprints.soton.ac.uk/id/eprint/56177
ISSN: 0953-816X
PURE UUID: f1b6d2aa-3a37-494e-b8f0-79cb06f7514e

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Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:31

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Contributors

Author: F. Docagne
Author: S.J. Campbell
Author: A.F. Bristow
Author: S. Poole
Author: S. Vigues
Author: C. Guaza
Author: V.H. Perry
Author: D.C. Anthony

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