c-Myc represses the murine Nramp I promoter
c-Myc represses the murine Nramp I promoter
The Nramp1 (natural resistance-associated macrophage protein 1) gene modulates the growth of intracellular pathogens and encodes a divalent cation transporter within lysosomes/late endosomes of macrophages. Nramp1 modulates the cytoplasmic iron pool. Wu, Polack and Dalla-Favera [(1999) Science 283, 676–679] showed reciprocal control of H-ferritin and IRP2 by c-Myc, and suggest that c-Myc regulates genes to increase cytoplasmic iron. A role for c-Myc in Nramp1 regulation was evaluated. Co-transfection studies show that c-Myc represses Nramp1 promoter function. Five non-canonical Myc-max binding sites (E-box) identified within the Nramp1 5´-flanking sequence are not responsible for the inhibitory effects of c-Myc on Nramp1 expression. An initiator(s) adjacent to the transcription-initiation site is a candidate for the inhibition observed. Results are consistent with a role for Nramp1 removing iron from the cytosol and antagonizing c-Myc function.
774-777
Bowen, H.
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Biggs, T.E.
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Baker, S.T.
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Phillips, E.
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Perry, V.H.
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Mann, D.A.
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Barton, C.H.
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1 August 2002
Bowen, H.
4ed2a24f-bf81-4d60-b475-567f9ad321bb
Biggs, T.E.
5ef9af49-c9ba-44ed-b9b1-2aacdc5bee2d
Baker, S.T.
359e3325-2545-44e5-a64d-8d4a433d8af7
Phillips, E.
66ddd4cb-19a2-4d08-889b-12f418e6878b
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
Mann, D.A.
54e772bb-f94f-4485-98c8-b2339a929d86
Barton, C.H.
5dfb4e1a-d559-4b58-9036-31de1e6c9ad8
Bowen, H., Biggs, T.E., Baker, S.T., Phillips, E., Perry, V.H., Mann, D.A. and Barton, C.H.
(2002)
c-Myc represses the murine Nramp I promoter.
Biochemical Society Transactions, 30 (4), .
Abstract
The Nramp1 (natural resistance-associated macrophage protein 1) gene modulates the growth of intracellular pathogens and encodes a divalent cation transporter within lysosomes/late endosomes of macrophages. Nramp1 modulates the cytoplasmic iron pool. Wu, Polack and Dalla-Favera [(1999) Science 283, 676–679] showed reciprocal control of H-ferritin and IRP2 by c-Myc, and suggest that c-Myc regulates genes to increase cytoplasmic iron. A role for c-Myc in Nramp1 regulation was evaluated. Co-transfection studies show that c-Myc represses Nramp1 promoter function. Five non-canonical Myc-max binding sites (E-box) identified within the Nramp1 5´-flanking sequence are not responsible for the inhibitory effects of c-Myc on Nramp1 expression. An initiator(s) adjacent to the transcription-initiation site is a candidate for the inhibition observed. Results are consistent with a role for Nramp1 removing iron from the cytosol and antagonizing c-Myc function.
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Published date: 1 August 2002
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Local EPrints ID: 56214
URI: http://eprints.soton.ac.uk/id/eprint/56214
ISSN: 0300-5127
PURE UUID: d0fc137a-eb70-4dba-8f00-d1544ec60b37
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Date deposited: 06 Aug 2008
Last modified: 09 Jan 2022 03:01
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Author:
H. Bowen
Author:
T.E. Biggs
Author:
S.T. Baker
Author:
E. Phillips
Author:
D.A. Mann
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