The University of Southampton
University of Southampton Institutional Repository

Bicuculline enhances the late GABAB receptor-mediated paired-pulse inhibition observed in rat hippocampal slices

Stanford, Ian M., Wheal, Howard V. and Chad, John E. (1995) Bicuculline enhances the late GABAB receptor-mediated paired-pulse inhibition observed in rat hippocampal slices European Journal of Pharmacology, 277, (2-3), pp. 229-234. (doi:10.1016/0014-2999(95)00083-W).

Record type: Article


The inhibition of CA1 pyramidal neurones in rat hippocampal slices was studied using extracellular recordings of population spike potential responses to paired orthodromic stimulation. Variation of the interpulse interval allowed the separation of an early phase of inhibition (interpulse interval 5–20 ms), blocked by the GABAA receptor antagonist bicuculline (1 ?M; n = 11), and a late phase (interpulse interval 200–400 ms) blocked by the GABAB receptor antagonist phaclofen (1 mM; n = 5) but enhanced by bicuculline (n = 11). Similar enhancement was not observed when conditioning response amplitudes were increased by increasing the stimulus strength, rather than bicuculline. Orthodromic stimulation leads to synaptic excitation of both pyramidal neurones and inhibitory interneurones, and may also lead to activation of inhibitory inputs onto interneurones. Bicuculline could prevent inhibition of the interneurones, and hence enhance the late, GABAB receptor-mediated inhibition. Conversely, the therapeutic administration of benzodiazepines would be postulated to enhance the inhibition of inhibitory interneurones, leading to an iatrogenic decrease in GABAB receptor-mediated inhibition.

Full text not available from this repository.

More information

Published date: 24 April 1995
Keywords: GABA receptor, hippocampus, epilepsy, synaptic inhibition, disinhibition


Local EPrints ID: 56248
ISSN: 0014-2999
PURE UUID: 631c4ef9-70c7-4c2a-81ab-64eab72bc00f
ORCID for John E. Chad: ORCID iD

Catalogue record

Date deposited: 22 Aug 2008
Last modified: 17 Jul 2017 14:31

Export record



Author: Ian M. Stanford
Author: Howard V. Wheal
Author: John E. Chad ORCID iD

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.