NADPH-diaphorase histochemistry in the terminal abdominal ganglion of the crayfish
NADPH-diaphorase histochemistry in the terminal abdominal ganglion of the crayfish
Nitric oxide (NO) has an important modulatory role on the processing of sensory signals in vertebrates and invertebrates. In this investigation we studied the potential sources of NO in the terminal abdominal ganglion of the crayfish, Pacifastacus leniusculus, using NADPH-diaphorase (NADPHd) histochemistry, with NADPHd acting as a marker for NO synthase (NOS). In the terminal ganglion a mean of 27 strongly labelled NADPHd-positive cell bodies were found, and of these 80% [of stained cell bodies] [corrected] occurred in three regions located in antero-lateral, central and posterior parts of the ganglion. Ventral and antero-ventral commissures as well as specific dorsal and ventral areas of the dendritic neuropil showed positive staining. Intense labelling was seen in the ventro-medial tract, and in the connective between the terminal ganglion and the 5th abdominal ganglion. In addition, some motor neurones and neurones with branches in the sensory commissures were NADPHd positive. Our finding that NADPHd-positive cells occur in consistent patterns in the terminal abdominal ganglion implies that NO may have a role in mechanosensory processing in the crayfish.
nitric oxide synthase, nitric oxide, NADPH diaphorase, histochemistry, pacifastacus leniusculus (crustacea)
289-299
Schuppe, Hansjürgen
17f82c3c-51da-46ff-a457-80d4f991b4ef
Aonuma, Hitoshi
a8b20ebb-d63c-4bd6-8a58-b0773ddaae10
Newland, Philip
7a018c0e-37ba-40f5-bbf6-49ab0f299dbb
January 2001
Schuppe, Hansjürgen
17f82c3c-51da-46ff-a457-80d4f991b4ef
Aonuma, Hitoshi
a8b20ebb-d63c-4bd6-8a58-b0773ddaae10
Newland, Philip
7a018c0e-37ba-40f5-bbf6-49ab0f299dbb
Schuppe, Hansjürgen, Aonuma, Hitoshi and Newland, Philip
(2001)
NADPH-diaphorase histochemistry in the terminal abdominal ganglion of the crayfish.
Cell and Tissue Research, 303 (2), .
(doi:10.1007/s004410000319).
Abstract
Nitric oxide (NO) has an important modulatory role on the processing of sensory signals in vertebrates and invertebrates. In this investigation we studied the potential sources of NO in the terminal abdominal ganglion of the crayfish, Pacifastacus leniusculus, using NADPH-diaphorase (NADPHd) histochemistry, with NADPHd acting as a marker for NO synthase (NOS). In the terminal ganglion a mean of 27 strongly labelled NADPHd-positive cell bodies were found, and of these 80% [of stained cell bodies] [corrected] occurred in three regions located in antero-lateral, central and posterior parts of the ganglion. Ventral and antero-ventral commissures as well as specific dorsal and ventral areas of the dendritic neuropil showed positive staining. Intense labelling was seen in the ventro-medial tract, and in the connective between the terminal ganglion and the 5th abdominal ganglion. In addition, some motor neurones and neurones with branches in the sensory commissures were NADPHd positive. Our finding that NADPHd-positive cells occur in consistent patterns in the terminal abdominal ganglion implies that NO may have a role in mechanosensory processing in the crayfish.
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Published date: January 2001
Keywords:
nitric oxide synthase, nitric oxide, NADPH diaphorase, histochemistry, pacifastacus leniusculus (crustacea)
Organisations:
Biological Sciences
Identifiers
Local EPrints ID: 56251
URI: http://eprints.soton.ac.uk/id/eprint/56251
ISSN: 0302-766X
PURE UUID: 64a8a0fa-01f2-4cb1-80f1-b0515403e582
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Date deposited: 08 Aug 2008
Last modified: 16 Mar 2024 02:59
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Author:
Hansjürgen Schuppe
Author:
Hitoshi Aonuma
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