The University of Southampton
University of Southampton Institutional Repository

Transcriptional control of Nramp 1: a paradigm for the repressive action of c-Myc

Record type: Article

Slc11a1/Nramp1 (solute carrier family 11 member a1/murine natural resistance-associated macrophage protein 1 gene) encodes a divalent cation transporter that resides within lysosomes/late endosomes of macrophages. Nramp1 modulates the cellular distribution of divalent cations in response to cell activation by intracellular pathogens. Nramp1 expression is repressed and activated by the proto-oncogene c-Myc and Miz-1 (c-Myc-interacting zinc finger protein 1) respectively. Here we demonstrate, using a c-Myc mutant (V394D, Val394Asp) that is incapable of binding Miz-1, that c-Myc repression of Nramp1 transcription is dependent on its interaction with Miz-1. An oligo pull-down assay demonstrates specific binding of recombinant Miz-1 to the Nramp1 Miz-1-binding site or initiator element(s), and Miz-1-dependent c-Myc recruitment.

Full text not available from this repository.

Citation

Lapham, A.S., Phillips, E.S. and Barton, C.H. (2004) Transcriptional control of Nramp 1: a paradigm for the repressive action of c-Myc Biochemical Society Transactions, 32, (6), pp. 1084-1086.

More information

Published date: 1 December 2004

Identifiers

Local EPrints ID: 56294
URI: http://eprints.soton.ac.uk/id/eprint/56294
ISSN: 0300-5127
PURE UUID: 518d5eb3-80bf-4407-adc6-456197120e15
ORCID for E.S. Phillips: ORCID iD orcid.org/0000-0001-5268-4203

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 17 Jul 2017 14:31

Export record

Contributors

Author: A.S. Lapham
Author: E.S. Phillips ORCID iD
Author: C.H. Barton

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×