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Characterization of a putative nitric oxide synthase in the neuromuscular system of the parasitic nematode, Ascaris suum

Characterization of a putative nitric oxide synthase in the neuromuscular system of the parasitic nematode, Ascaris suum
Characterization of a putative nitric oxide synthase in the neuromuscular system of the parasitic nematode, Ascaris suum
In this paper we report on the biochemical presence of nitric oxide synthase (NOS)-like activity in Ascaris suum tissue and examine the pharmacological effect of NO donors on A. suum muscle strip preparation. NOS activity was determined by monitoring the formation of [3H]citrulline from [3H]L-arginine and NO formation via the oxyhaemoglobin assay. Neuromuscular tissue from A. suum which stained positively for NADPH diaphorase, contained NOS activity. Neither NOS activity nor NADPH diaphorase staining was detected in intestinal tissue. The absence of Ca2+, NADPH and other co-factors normally required for mammalian neuronal NOS activity only partially reduced the formation of both citrulline and NO by A. suum neuromuscular homogenate. The results of the biochemical assays indicate the presence of an enzyme capable of producing NO and citrulline, but with a different profile from that of rat neuronal NOS. We also present preliminary evidence for the action of NO (NO donors) in the neuromuscular system of A. suum.
0031-1820
219-232
Bascal, Z.A.
de4b5c80-c092-4bd5-977b-d526f69d6081
Cunningham, J.M.
7064cd6c-6574-40a2-9613-d2afb21a14c6
Holden-Dye, L.
8032bf60-5db6-40cb-b71c-ddda9d212c8e
Shea, M.
0d528cb7-7222-4657-8fe4-3c535a04cfd6
Walker, R.J.
b6597591-587e-488a-8a54-89156c42ce8d
Bascal, Z.A.
de4b5c80-c092-4bd5-977b-d526f69d6081
Cunningham, J.M.
7064cd6c-6574-40a2-9613-d2afb21a14c6
Holden-Dye, L.
8032bf60-5db6-40cb-b71c-ddda9d212c8e
Shea, M.
0d528cb7-7222-4657-8fe4-3c535a04cfd6
Walker, R.J.
b6597591-587e-488a-8a54-89156c42ce8d

Bascal, Z.A., Cunningham, J.M., Holden-Dye, L., Shea, M. and Walker, R.J. (2001) Characterization of a putative nitric oxide synthase in the neuromuscular system of the parasitic nematode, Ascaris suum. Parasitology, 122 (2), 219-232. (doi:10.1017/S003118200100720X).

Record type: Article

Abstract

In this paper we report on the biochemical presence of nitric oxide synthase (NOS)-like activity in Ascaris suum tissue and examine the pharmacological effect of NO donors on A. suum muscle strip preparation. NOS activity was determined by monitoring the formation of [3H]citrulline from [3H]L-arginine and NO formation via the oxyhaemoglobin assay. Neuromuscular tissue from A. suum which stained positively for NADPH diaphorase, contained NOS activity. Neither NOS activity nor NADPH diaphorase staining was detected in intestinal tissue. The absence of Ca2+, NADPH and other co-factors normally required for mammalian neuronal NOS activity only partially reduced the formation of both citrulline and NO by A. suum neuromuscular homogenate. The results of the biochemical assays indicate the presence of an enzyme capable of producing NO and citrulline, but with a different profile from that of rat neuronal NOS. We also present preliminary evidence for the action of NO (NO donors) in the neuromuscular system of A. suum.

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Published date: 1 February 2001

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Local EPrints ID: 56296
URI: https://eprints.soton.ac.uk/id/eprint/56296
ISSN: 0031-1820
PURE UUID: 9dcefc07-ed2c-4b13-8911-4ae24759198a

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Date deposited: 08 Aug 2008
Last modified: 13 Mar 2019 20:35

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