Functional effects of the chronic expression of IL-1 beta in the striatum
Functional effects of the chronic expression of IL-1 beta in the striatum
Interleukin-1 beta (IL-1) is associated with a spectrum of inflammatory processes related to neurodegenerative disease. The effects of single-bolus injections of IL-1 into the CNS parenchyma give rise to neutrophil (PMN) recruitment, transient blood–brain barrier (BBB) breakdown, but no overt damage to CNS integrity. The effects of long-term IL-1 expression in brain parenchyma on CNS integrity have not been investigated. We used a recombinant adenovirus expressing IL-1 (AdIL-1) to examine the chronic effects of IL-1 expression in the CNS. Histochemical and immunohistochemical methods were used to characterize the immune response to an intrastriatal injection of 107 pfu of AdIL-1 and ELISA to quantified the IL-1 protein produced by the adenovector. IL-1 expression was detectable at 4, 8, 14, and 30 days postinjection. At days 1 and 2, some monocyte adherence was observed in the lumen of the vessels but between days 8 and 14 there was marked and restricted recruitment of PMNs, vasodilatation and breakdown of the BBB. Microglia and astrocyte activation was evident at days 2, 8 and 14. At days 8 and 14, disorganization of the nervous tissue, demyelination were observed. The number of neurons in the striatum was not affected by the treatment. At 30 days, the nervous tissue appeared normal with no signs of demyelination, infiltration or BBB breakdown. Thus, we show that chronic expression of IL-1 can damage CNS integrity, that IL-1 generates a restricted leukocyte recruitment profile in the CNS, and that there is no tachyphylaxis to chronic IL-1 expression
demyelination, IL-1, neuroinflammation
160-160
Ferrari, C.
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Prada, F.
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Muraro, N.
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Depino, A.
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Perry, V.H.
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Anthony, D.C.
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Pitossi, F.
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1 December 2003
Ferrari, C.
40cd22e5-0daf-43d5-b62f-8dfc236ea30f
Prada, F.
fbbd9a1e-8f08-421a-a84f-2fa1e4b80e2d
Muraro, N.
5c6b28b2-6e66-4a23-91d9-9a6896af9621
Depino, A.
fa53e09d-6406-4aa6-a5c7-1a5428429864
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
Anthony, D.C.
70fb8e27-4e74-4c72-b1b5-3a4ca0d6b8cf
Pitossi, F.
9f077ae6-4ca9-4cb5-8636-8da535e103f2
Ferrari, C., Prada, F., Muraro, N., Depino, A., Perry, V.H., Anthony, D.C. and Pitossi, F.
(2003)
Functional effects of the chronic expression of IL-1 beta in the striatum.
Journal of Neurochemistry, 87 (Supplement 1), .
(doi:10.1046/j.1474-1644.2003.2175_15.x).
Abstract
Interleukin-1 beta (IL-1) is associated with a spectrum of inflammatory processes related to neurodegenerative disease. The effects of single-bolus injections of IL-1 into the CNS parenchyma give rise to neutrophil (PMN) recruitment, transient blood–brain barrier (BBB) breakdown, but no overt damage to CNS integrity. The effects of long-term IL-1 expression in brain parenchyma on CNS integrity have not been investigated. We used a recombinant adenovirus expressing IL-1 (AdIL-1) to examine the chronic effects of IL-1 expression in the CNS. Histochemical and immunohistochemical methods were used to characterize the immune response to an intrastriatal injection of 107 pfu of AdIL-1 and ELISA to quantified the IL-1 protein produced by the adenovector. IL-1 expression was detectable at 4, 8, 14, and 30 days postinjection. At days 1 and 2, some monocyte adherence was observed in the lumen of the vessels but between days 8 and 14 there was marked and restricted recruitment of PMNs, vasodilatation and breakdown of the BBB. Microglia and astrocyte activation was evident at days 2, 8 and 14. At days 8 and 14, disorganization of the nervous tissue, demyelination were observed. The number of neurons in the striatum was not affected by the treatment. At 30 days, the nervous tissue appeared normal with no signs of demyelination, infiltration or BBB breakdown. Thus, we show that chronic expression of IL-1 can damage CNS integrity, that IL-1 generates a restricted leukocyte recruitment profile in the CNS, and that there is no tachyphylaxis to chronic IL-1 expression
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Published date: 1 December 2003
Additional Information:
P15-33
Keywords:
demyelination, IL-1, neuroinflammation
Identifiers
Local EPrints ID: 56328
URI: http://eprints.soton.ac.uk/id/eprint/56328
ISSN: 0022-3042
PURE UUID: be7ff1f9-55a2-4435-a269-c2a09da168d1
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Date deposited: 06 Aug 2008
Last modified: 15 Mar 2024 11:01
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Contributors
Author:
C. Ferrari
Author:
F. Prada
Author:
N. Muraro
Author:
A. Depino
Author:
D.C. Anthony
Author:
F. Pitossi
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