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Integrin alpha(2)-deficient mice develop normally, are fertile, but display partially defective platelet interaction with collagen

Holtkotter, O., Nieswandt, B., Smyth, N., Muller, M., Hafner, M., Schulte, V., Krieg, T. and Eckes, B. (2002) Integrin alpha(2)-deficient mice develop normally, are fertile, but display partially defective platelet interaction with collagen The Journal of Biological Chemistry, 277, (13), pp. 10789-10794. (doi:10.1074/jbc.M112307200).

Record type: Article

Abstract

The integrin 2-subunit was ablated in mice by targeted deletion of the ITGA2 gene. 2-Deficient animals develop normally, are fertile, and reproduce. Surprisingly, no obvious anatomical or histological differences were observed in mutant mice. Besides its significance in tissue morphogenesis, integrin 21 has been reported to play a major role in hemostasis by mediating platelet adhesion and activation on subendothelial collagen. To define its role in hemostasis, 2-deficient platelets were analyzed for their capacity to adhere to and aggregate in response to fibrillar or soluble collagen type I. We show that aggregation of 2-deficient platelets to fibrillar collagen is delayed but not reduced, whereas aggregation to enzymatically digested soluble collagen is abolished. Furthermore, 2-deficient platelets normally adhere to fibrillar collagen. However, in the presence of an antibody against GPVI (activating platelet collagen receptor), adhesion of 2-deficient but not wild type platelets is abrogated. These results demonstrate that integrin 21 significantly contributes to platelet adhesion to (fibrillar) collagen, which is further confirmed by the abolished adhesion of 2-deficient platelets to soluble collagen. Thus, 21 plays a supportive rather than an essential role in platelet-collagen interactions. These results are in agreement with the observation that 21-deficient animals suffer no bleeding anomalies.

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Published date: 1 March 2002

Identifiers

Local EPrints ID: 56332
URI: http://eprints.soton.ac.uk/id/eprint/56332
ISSN: 0021-9258
PURE UUID: 9b972794-33fb-4daa-abd0-7854a4f5c4a3

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Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:31

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Contributors

Author: O. Holtkotter
Author: B. Nieswandt
Author: N. Smyth
Author: M. Muller
Author: M. Hafner
Author: V. Schulte
Author: T. Krieg
Author: B. Eckes

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