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DDR1-deficient mice show localized subepithelial GBM thickening with focal loss of slit diaphragms and proteinuria

DDR1-deficient mice show localized subepithelial GBM thickening with focal loss of slit diaphragms and proteinuria
DDR1-deficient mice show localized subepithelial GBM thickening with focal loss of slit diaphragms and proteinuria
DDR1-deficient mice show localized subepithelial GBM thickening with focal loss of slit diaphragms and proteinuria.
Background Type IV collagen in basement membranes is a ligand for the receptor tyrosine kinase discoidin domain receptor 1 (DDR1). DDR1 is expressed in renal cells and regulates cell adhesion and proliferation ex vivo. The interaction between type IV collagen and cell surface receptors is believed important for normal renal function as well as significant in chronic renal diseases and we therefore analyzed mice with a targeted deletion of DDR1.
Methods Homozygous DDR1 knockout mice were compared to heterozygous and wild-type animals. The quantitative and qualitative amount of proteinuria was measured by urine-microelectrophoresis. Structural changes of the kidneys were determined by immunohistochemistry, light microscopy, and electron microscopy.
Results Compared to heterozygous littermates, adult DDR1 knockout mice showed a selective middle- to high-molecular proteinuria of up to 0.3 g/L and urinary acanthocytes. There was no evidence of uremia with no change in serum urea in the first 9 months of age. Little apparent change in renal morphology was detected using light microscopy. However, electron microscopy showed a localized, subepithelial, mushroom-like isodense thickening of the glomerular basement membrane (GBM). Within these areas, a focal loss of the podocytic slit diaphragms occurred.
Conclusion The loss of cell-matrix communication in DDR1-deficient podocytes appears to result in excess synthesis of basement membrane proteins leading to disturbed anchorage of foot processes and disruption of the slit diaphragm. Our data suggest that the interaction between type IV collagen and DDR1 plays an important role in maintaining the structural integrity of the GBM.
collagen receptors, discoidin domain receptor, glomerular basement membrane, type IV collagen, extracellular matrix, proteinuria, tyrosine kinase receptor
0085-2538
102-111
Gross, O.
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Beirowski, B.
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Harvey, S.J.
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McFadden, C.
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Chen, D.
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Tam, S.
33d1e2b0-176d-43a2-a6ec-9333f70b8b23
Thorner, P.S.
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Smyth, N.
0eba2a40-3b43-4d40-bb64-621bd7e9d505
Addicks, K.
4e57ba3a-7a1c-403d-9b37-0fdab7965bf6
Bloch, W.
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Ninomiya, Y.
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Sado, Y.
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Weber, M.
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Vogel, W.F.
ab87260f-1814-41b3-870f-5597bc506844
Gross, O.
59c91c49-5a0d-4cb2-9120-7b192d5df3f0
Beirowski, B.
470c9752-1a01-40d1-83db-bd898cb7db00
Harvey, S.J.
54ab3675-e586-400a-91aa-99e7bed5451b
McFadden, C.
14c4d158-3865-4456-9697-85468a187d7a
Chen, D.
52c82b57-6894-41ce-b79b-d7448bcf0f66
Tam, S.
33d1e2b0-176d-43a2-a6ec-9333f70b8b23
Thorner, P.S.
34f83e0a-1a27-4660-bc48-6b4ea2d7da16
Smyth, N.
0eba2a40-3b43-4d40-bb64-621bd7e9d505
Addicks, K.
4e57ba3a-7a1c-403d-9b37-0fdab7965bf6
Bloch, W.
d6624bb0-f7ce-4979-9780-a6290bb2ac8f
Ninomiya, Y.
89d6577b-b762-4607-9ca9-564e9c341108
Sado, Y.
f230bb24-b715-4bc9-84f6-a8a5aaa5c42f
Weber, M.
9d3b3715-fde2-4207-9315-f26ed61ddd71
Vogel, W.F.
ab87260f-1814-41b3-870f-5597bc506844

Gross, O., Beirowski, B., Harvey, S.J., McFadden, C., Chen, D., Tam, S., Thorner, P.S., Smyth, N., Addicks, K., Bloch, W., Ninomiya, Y., Sado, Y., Weber, M. and Vogel, W.F. (2004) DDR1-deficient mice show localized subepithelial GBM thickening with focal loss of slit diaphragms and proteinuria. Kidney International, 66 (1), 102-111. (doi:10.1111/j.1523-1755.2004.00712.x).

Record type: Article

Abstract

DDR1-deficient mice show localized subepithelial GBM thickening with focal loss of slit diaphragms and proteinuria.
Background Type IV collagen in basement membranes is a ligand for the receptor tyrosine kinase discoidin domain receptor 1 (DDR1). DDR1 is expressed in renal cells and regulates cell adhesion and proliferation ex vivo. The interaction between type IV collagen and cell surface receptors is believed important for normal renal function as well as significant in chronic renal diseases and we therefore analyzed mice with a targeted deletion of DDR1.
Methods Homozygous DDR1 knockout mice were compared to heterozygous and wild-type animals. The quantitative and qualitative amount of proteinuria was measured by urine-microelectrophoresis. Structural changes of the kidneys were determined by immunohistochemistry, light microscopy, and electron microscopy.
Results Compared to heterozygous littermates, adult DDR1 knockout mice showed a selective middle- to high-molecular proteinuria of up to 0.3 g/L and urinary acanthocytes. There was no evidence of uremia with no change in serum urea in the first 9 months of age. Little apparent change in renal morphology was detected using light microscopy. However, electron microscopy showed a localized, subepithelial, mushroom-like isodense thickening of the glomerular basement membrane (GBM). Within these areas, a focal loss of the podocytic slit diaphragms occurred.
Conclusion The loss of cell-matrix communication in DDR1-deficient podocytes appears to result in excess synthesis of basement membrane proteins leading to disturbed anchorage of foot processes and disruption of the slit diaphragm. Our data suggest that the interaction between type IV collagen and DDR1 plays an important role in maintaining the structural integrity of the GBM.

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Published date: 1 July 2004
Keywords: collagen receptors, discoidin domain receptor, glomerular basement membrane, type IV collagen, extracellular matrix, proteinuria, tyrosine kinase receptor

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Local EPrints ID: 56349
URI: http://eprints.soton.ac.uk/id/eprint/56349
ISSN: 0085-2538
PURE UUID: 51123875-c39a-4912-a957-da0b3b18ab75

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Date deposited: 08 Aug 2008
Last modified: 08 Jan 2022 07:07

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Contributors

Author: O. Gross
Author: B. Beirowski
Author: S.J. Harvey
Author: C. McFadden
Author: D. Chen
Author: S. Tam
Author: P.S. Thorner
Author: N. Smyth
Author: K. Addicks
Author: W. Bloch
Author: Y. Ninomiya
Author: Y. Sado
Author: M. Weber
Author: W.F. Vogel

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