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Stabilisation of TG- and AG-containing antiparallel DNA triplexes by tripler-binding ligands

Stabilisation of TG- and AG-containing antiparallel DNA triplexes by tripler-binding ligands
Stabilisation of TG- and AG-containing antiparallel DNA triplexes by tripler-binding ligands
We have used DNase I footprinting to examine the interaction of several triplex-binding ligands with antiparallel TG- and AG-containing triplexes. We find that although a 17mer TG-containing oligonucleotide on its own fails to produce a footprint at concentrations as high as 30 µM, this interaction can be stabilised by several ligands. Within a series of disubstituted amidoanthraquinones we find that the 2,7- regioisomer affords the best stabilisation of this TG triplex, though the 1,8- isomer also stabilises this interaction to some extent. By contrast the 1,5- and 2,6- regioisomers show no interaction with TG triplexes. Similar studies with a 13mer AG-containing oligonucleotide show the opposite pattern of stabilisation: the 2,6- and 1,5- isomers stabilise this triplex, but the 2,7- and 1,8-compounds do not. The polycyclic compound BePI strongly stabilises TG- but not AG-containing triplexes, while a substituted naphthylquinoline interacts with both antiparallel triplex motifs.
0305-1048
1935-1942
Keppler, Melanie D.
6452c8eb-268a-47f4-955d-e1819df05333
Neidle, Stephen
edf2d7ee-a257-4d4c-a3ea-b3c9c42b5ff3
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Keppler, Melanie D.
6452c8eb-268a-47f4-955d-e1819df05333
Neidle, Stephen
edf2d7ee-a257-4d4c-a3ea-b3c9c42b5ff3
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f

Keppler, Melanie D., Neidle, Stephen and Fox, Keith R. (2001) Stabilisation of TG- and AG-containing antiparallel DNA triplexes by tripler-binding ligands. Nucleic Acids Research, 29 (9), 1935-1942.

Record type: Article

Abstract

We have used DNase I footprinting to examine the interaction of several triplex-binding ligands with antiparallel TG- and AG-containing triplexes. We find that although a 17mer TG-containing oligonucleotide on its own fails to produce a footprint at concentrations as high as 30 µM, this interaction can be stabilised by several ligands. Within a series of disubstituted amidoanthraquinones we find that the 2,7- regioisomer affords the best stabilisation of this TG triplex, though the 1,8- isomer also stabilises this interaction to some extent. By contrast the 1,5- and 2,6- regioisomers show no interaction with TG triplexes. Similar studies with a 13mer AG-containing oligonucleotide show the opposite pattern of stabilisation: the 2,6- and 1,5- isomers stabilise this triplex, but the 2,7- and 1,8-compounds do not. The polycyclic compound BePI strongly stabilises TG- but not AG-containing triplexes, while a substituted naphthylquinoline interacts with both antiparallel triplex motifs.

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Published date: May 2001

Identifiers

Local EPrints ID: 56357
URI: http://eprints.soton.ac.uk/id/eprint/56357
ISSN: 0305-1048
PURE UUID: c0813fec-9815-4025-b38a-6f498bdec4f9
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

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Date deposited: 08 Aug 2008
Last modified: 09 Jan 2022 02:35

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Contributors

Author: Melanie D. Keppler
Author: Stephen Neidle
Author: Keith R. Fox ORCID iD

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