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Auxin transport promotes Arabidopsis lateral root initiation

Auxin transport promotes Arabidopsis lateral root initiation
Auxin transport promotes Arabidopsis lateral root initiation
Lateral root development in Arabidopsis provides a model for the study of hormonal signals that regulate postembryonic organogenesis in higher plants. Lateral roots originate from pairs of pericycle cells, in several cell files positioned opposite the xylem pole, that initiate a series of asymmetric, transverse divisions. The auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) arrests lateral root development by blocking the first transverse division(s). We investigated the basis of NPA action by using a cell-specific reporter to demonstrate that xylem pole pericycle cells retain their identity in the presence of the auxin transport inhibitor. However, NPA causes indoleacetic acid (IAA) to accumulate in the root apex while reducing levels in basal tissues critical for lateral root initiation. This pattern of IAA redistribution is consistent with NPA blocking basipetal IAA movement from the root tip. Characterization of lateral root development in the shoot meristemless1 mutant demonstrates that root basipetal and leaf acropetal auxin transport activities are required during the initiation and emergence phases, respectively, of lateral root development.
1040-4651
843-852
Casimiro, Ilda
bb668bc2-bf37-4d44-8dc1-7a771f497bf1
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Bhalerao, Rishikesh P.
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Beeckman, Tom
b7467556-ba35-4f33-8964-9403629a825f
Dhooge, Sandra
6cef5352-9ad7-4c9e-8aa3-ec05c9d6810f
Swarup, Ranjan
01a7c8f9-9165-4aa8-8d7f-f84879894f97
Graham, Neil
1c2d7e5c-9dcb-4e23-8fcd-50f6584d2d7e
Inze, Dirk
8b6ced31-0f09-46aa-86b6-836e2c8f1659
Sandberg, Goran
5807cf0b-1e87-47d5-bd3f-d67f6ac83bf9
Casero, Pedro J.
91fd1fc9-2232-484f-8fb5-9c1e562bb960
Bennett, Malcolm
1c8bd66c-2a22-49f0-a8e6-baebb9b7d42c
Casimiro, Ilda
bb668bc2-bf37-4d44-8dc1-7a771f497bf1
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Bhalerao, Rishikesh P.
7d29fc1b-920b-4f81-b805-14b4a45b7bb0
Beeckman, Tom
b7467556-ba35-4f33-8964-9403629a825f
Dhooge, Sandra
6cef5352-9ad7-4c9e-8aa3-ec05c9d6810f
Swarup, Ranjan
01a7c8f9-9165-4aa8-8d7f-f84879894f97
Graham, Neil
1c2d7e5c-9dcb-4e23-8fcd-50f6584d2d7e
Inze, Dirk
8b6ced31-0f09-46aa-86b6-836e2c8f1659
Sandberg, Goran
5807cf0b-1e87-47d5-bd3f-d67f6ac83bf9
Casero, Pedro J.
91fd1fc9-2232-484f-8fb5-9c1e562bb960
Bennett, Malcolm
1c8bd66c-2a22-49f0-a8e6-baebb9b7d42c

Casimiro, Ilda, Marchant, Alan, Bhalerao, Rishikesh P., Beeckman, Tom, Dhooge, Sandra, Swarup, Ranjan, Graham, Neil, Inze, Dirk, Sandberg, Goran, Casero, Pedro J. and Bennett, Malcolm (2001) Auxin transport promotes Arabidopsis lateral root initiation. The Plant Cell, 13, 843-852.

Record type: Article

Abstract

Lateral root development in Arabidopsis provides a model for the study of hormonal signals that regulate postembryonic organogenesis in higher plants. Lateral roots originate from pairs of pericycle cells, in several cell files positioned opposite the xylem pole, that initiate a series of asymmetric, transverse divisions. The auxin transport inhibitor N-1-naphthylphthalamic acid (NPA) arrests lateral root development by blocking the first transverse division(s). We investigated the basis of NPA action by using a cell-specific reporter to demonstrate that xylem pole pericycle cells retain their identity in the presence of the auxin transport inhibitor. However, NPA causes indoleacetic acid (IAA) to accumulate in the root apex while reducing levels in basal tissues critical for lateral root initiation. This pattern of IAA redistribution is consistent with NPA blocking basipetal IAA movement from the root tip. Characterization of lateral root development in the shoot meristemless1 mutant demonstrates that root basipetal and leaf acropetal auxin transport activities are required during the initiation and emergence phases, respectively, of lateral root development.

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Published date: April 2001

Identifiers

Local EPrints ID: 56362
URI: https://eprints.soton.ac.uk/id/eprint/56362
ISSN: 1040-4651
PURE UUID: 9ff55df4-85dd-405e-870a-e2dfa78f0f67

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Date deposited: 08 Aug 2008
Last modified: 13 Mar 2019 20:35

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Contributors

Author: Ilda Casimiro
Author: Alan Marchant
Author: Rishikesh P. Bhalerao
Author: Tom Beeckman
Author: Sandra Dhooge
Author: Ranjan Swarup
Author: Neil Graham
Author: Dirk Inze
Author: Goran Sandberg
Author: Pedro J. Casero
Author: Malcolm Bennett

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