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Ras/Erk signaling is essential for activation of protein synthesis by Gq protein receptor agonists in adult cardiomyocytes

Record type: Article

The Gq protein-coupled receptor agonists phenylephrine (PE) and endothelin-1 (ET-1) induce cardiac hypertrophy and stimulate protein synthesis in cardiomyocytes. This study aims to investigate how they activate mRNA translation in adult cardiomyocytes. PE and ET-1 do not activate protein kinase B but stimulate Ras and Erk, and their ability to activate protein synthesis was blocked by inhibition of Ras or MEK and by rapamycin, which inhibits mTOR (mammalian target of rapamycin). These agonists activated ribosomal protein S6 kinase 1 (S6K1) and induced phosphorylation of eIF4E-binding protein-1 (4E-BP1) and its release from eIF4E. These effects were blocked by inhibitors of MEK. Furthermore, adenovirus-mediated expression of constitutively-active MEK1 caused activation of S6K1, phosphorylation of 4E-BP1, and activation of protein synthesis in a rapamycin-sensitive manner. Expression of N17Ras inhibited the regulation of S6K1 and protein synthesis by GqPCR agonists. These data point to a signaling pathway involving Ras and MEK that acts, with mTOR, to control regulatory translation factors and activate protein synthesis. This study provides new insights into the mechanisms underlying the stimulation of protein synthesis by hypertrophic agents in heart.

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Citation

Wang, L.J. and Proud, C.G. (2002) Ras/Erk signaling is essential for activation of protein synthesis by Gq protein receptor agonists in adult cardiomyocytes Circulation Research, 91, pp. 821-829. (doi:10.1161/01.RES.0000041029.97988.E9).

More information

Published date: 1 November 2002
Keywords: cardiac hypertrophy, MEK, mRNA translation, S6 kinase 1, eIF4E-binding protein-1

Identifiers

Local EPrints ID: 56388
URI: http://eprints.soton.ac.uk/id/eprint/56388
ISSN: 0009-7330
PURE UUID: 5566d664-5138-4d4a-b074-0c963a8de593

Catalogue record

Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:30

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Contributors

Author: L.J. Wang
Author: C.G. Proud

University divisions


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