Lillycrop, K.A., Jackson, A.A., Hanson, M.A. and Burdge, G.C.
The effect of dietary protein restriction in pregnant rats on the expression of DNA methyltransferases and methyl CpG binding protein 2 in the liver after weaning.
Proceedings of the Nutrition Society, 65, (OCA-B) (doi:10.1017/S002966510600526X).
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Induction of a modified metabolic phenotype in the offspring by feeding a protein-restricted (PR) diet during pregnancy in the rat involves DNA hypomethylation and altered covalent histone modifications leading to increased expression of specific genes (Lillycrop et al. 2005a,b). Hypomethylation of gene promoters may be achieved by impaired DNA methylation de novo, loss of CpG methylation during mitosis, or active demethylation. Histone modifications which modulate transcription involve binding of methyl CpG binding protein (MeCP)-2 to methylated DNA and recruitment of histone-modifying enzymes (Bird, 2002). We investigated in the offspring the effect of feeding a PR diet during pregnancy on the expression of hepatic DNA methyltransferase (DMNT) 1 which maintains CpG methylation, DNMT 3a and 3b which catalyse DNA methylation de novo and the DNA demethylase MBD2.
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