Sequence effects of single base loops in intramolecular quadruplex DNA
Sequence effects of single base loops in intramolecular quadruplex DNA
We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1?,2?-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2°C). Quadruplexes containing loops with single A residues were less stable by 8°C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.
dna quadruplex, dna structure
1657-1660
Rachwal, Phillip A.
8bd3be31-babc-456b-840e-6447b57c6999
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
17 April 2007
Rachwal, Phillip A.
8bd3be31-babc-456b-840e-6447b57c6999
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Rachwal, Phillip A., Brown, Tom and Fox, Keith R.
(2007)
Sequence effects of single base loops in intramolecular quadruplex DNA.
FEBS Letters, 581 (8), .
(doi:10.1016/j.febslet.2007.03.040).
Abstract
We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1?,2?-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2°C). Quadruplexes containing loops with single A residues were less stable by 8°C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.
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Submitted date: 7 February 2007
Published date: 17 April 2007
Keywords:
dna quadruplex, dna structure
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Local EPrints ID: 56440
URI: http://eprints.soton.ac.uk/id/eprint/56440
ISSN: 0014-5793
PURE UUID: 22e29e91-3956-4cee-82ec-190f62fa5e34
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Date deposited: 07 Aug 2008
Last modified: 16 Mar 2024 02:36
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Author:
Phillip A. Rachwal
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