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Analysis of mTOR signaling by the small G-proteins, Rheb and RhebL1

Analysis of mTOR signaling by the small G-proteins, Rheb and RhebL1
Analysis of mTOR signaling by the small G-proteins, Rheb and RhebL1
The small G protein Rheb (Ras homologue enriched in brain) is known to promote mammalian target of rapamycin (mTOR) signaling. In this study, we show that Rheb like-1 protein (RhebL1) rescues mTOR signaling during nutrient withdrawal and that tuberous sclerosis complex-1 (TSC) and TSC2 impairs RhebL1-mediated signaling through mTOR. We identify critical residues within the switch I region (N41) and ‘constitutive’ effector (Ec) region (Y/F54 and L56) of Rheb and RhebL1, which are required for their efficient activation of mTOR signaling. Mutation of Rheb and RhebL1 at N41 impaired their interaction with mTOR, which identifies mTOR as a common downstream target of both Rheb and RhebL1.
Rheb, mTOR, TSC2, S6K1, 4E-BP1, RhebL1
0014-5793
4763-4768
Tee, Andrew R.
01508d8d-e02a-4943-99b6-0680aee4f26d
Blenis, John
113180f3-68e7-48b0-8bfb-800c178e2871
Proud, Christopher G.
59dabfc8-4b44-4be8-a17f-578a58550cb3
Tee, Andrew R.
01508d8d-e02a-4943-99b6-0680aee4f26d
Blenis, John
113180f3-68e7-48b0-8bfb-800c178e2871
Proud, Christopher G.
59dabfc8-4b44-4be8-a17f-578a58550cb3

Tee, Andrew R., Blenis, John and Proud, Christopher G. (2005) Analysis of mTOR signaling by the small G-proteins, Rheb and RhebL1. Febs Letters, 579 (21), 4763-4768. (doi:10.1016/j.febslet.2005.07.054).

Record type: Article

Abstract

The small G protein Rheb (Ras homologue enriched in brain) is known to promote mammalian target of rapamycin (mTOR) signaling. In this study, we show that Rheb like-1 protein (RhebL1) rescues mTOR signaling during nutrient withdrawal and that tuberous sclerosis complex-1 (TSC) and TSC2 impairs RhebL1-mediated signaling through mTOR. We identify critical residues within the switch I region (N41) and ‘constitutive’ effector (Ec) region (Y/F54 and L56) of Rheb and RhebL1, which are required for their efficient activation of mTOR signaling. Mutation of Rheb and RhebL1 at N41 impaired their interaction with mTOR, which identifies mTOR as a common downstream target of both Rheb and RhebL1.

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More information

Submitted date: 10 March 2005
Published date: 29 August 2005
Keywords: Rheb, mTOR, TSC2, S6K1, 4E-BP1, RhebL1

Identifiers

Local EPrints ID: 56483
URI: https://eprints.soton.ac.uk/id/eprint/56483
ISSN: 0014-5793
PURE UUID: d1fe5a82-2b09-4d36-93e5-18856dcb0981

Catalogue record

Date deposited: 07 Aug 2008
Last modified: 17 Jul 2017 14:30

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